Abstract
The antioxidant potential of a protein hydrolysate depends on the composition and sequence of antioxidant peptides produced during hydrolysis and the interaction of these peptides with other oxidative compounds. There are few studies on the antioxidant potential of canola (Brassica napus L.); however, as a source rich in protein (30–40%), canola paste is suitable to produce hydrolysates with this function. The objective of this work was to obtain hydrolysates using the sequential pepsin-pancreatin (PP) system, to determine some chemical characteristics and their antioxidant potential. Limited hydrolysates with a degree of hydrolysis of less than 10 (DH < 10) were obtained for canola protein hydrolysates at 5 min of reaction (CPH5min) with 7.97% and for canola protein hydrolysates at 10 min of reaction (CPH10min) with 9.78%. Regarding its amino acid (AA) composition, a high proportion of the amino acid His was found with 21.1% for CPH5min and 22.8% for CPH10min, as well as other hydrophobic amino acids in lower concentrations. In electrophoresis, it was found that 2S napin and 12S cruciferin, typical of canola, were still present. The hydrolysates also presented a high content of phenolic compounds (CPH5min with 190 mg EAG/100 g and CPH10min with 220 mg EAG/100 g), which along with the antioxidant activity evaluated with DPPH radicals (CPH5min with 29.01% and CPH10min with 35.6%) and ABTS, (CPH5min 5.45 mM TEAC/g and CPH10min 5.45 mM TEAC/g) were higher compared to the canola protein concentrate (CPC) used as a substrate to obtain the hydrolysates.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.