Antioxidant Potential of Black, Green and Oolong Tea Methanol Extracts

Abstract

Degenerative diseases and chronic diseases are often caused by oxidative stress. Oxidative stress caused by free radicals. Antixodant as inhibitor are needed to prevent it which is one of antioxidant sources is tea. Tea processing generally produce various kinds of teas such as black, green and oolong tea. Tea processing affect the content of phenolic compounds. The aim of the research is to evaluate phytochemical content, total phenolic content of black tea, green tea and oolong tea extracts using catechin, quercetin, kaempferol, myricetin as standard, and to evaluate the antioxidative potency of black tea, green tea and oolong tea extracts compared to catechin, quercetin, kaempferol, myricetin. Phytochemical assay using modified Farnsworth method, the antioxidant activity were measured by by its 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity. Green tea extract contained highest phenolic and flavonoid. The highest antioxidant activity was green tea extract with IC50=0,487 μg/mL. Green tea extract content phenol and flavonoid are higher compared to the other extracts, green tea extract has the highest antioxidant activity.