Abstract
Sorbaria tomentosa,, commonly known as “Berre” is native to Himalaya and Hindukush range in Pakistan. Qualitative phytochemical screening as well as quantitative antioxidant potential of its ethanolic extract was evaluated. Antioxidant potential of the extract was determined using standard methods like DPPH, FRAP, total phenolics, total flavonoids, ABTS radical cation scavenging assay and β-carotene linoleic acid emulsion system. Cu(II) and Zn(II) chelates of ,,Sorbaria tomentosa,, were also prepared and their antioxidant potential was compared with the extract as well as with synthetic antioxidants (BHT and BHA). It was observed that ,,Sorbaria tomentosa,, is a good source of natural antioxidant that worked efficiently compared to respective chelates. Keeping in view less efficiency of chelated extracts, stabilization studies of sunflower oil were conducted with ethanolic extract (250, 500, 1000 ppm) of ,,Sorbaria tomentosa,,. Various parameters like PV, FFA and IV were estimated to evaluate stabilization of oil. ,,Sorbaria tomentosa,, extract (1000 ppm) showed almost same role during stabilization like BHA at ambient condition during a storage period of 45 days. Keywords: Sorbaria tomentosa, phytochemicals, antioxidant potential, chelation, stabilization
Highlights
Sorbaria tomentosa, commonly known as “Berre” is native to Himalaya and Hindukush range in Pakistan
Estimation of Peroxide Value (PV), Free Fatty Acid Value (FFA) and Iodine Value (IV) Peroxide value, free fatty acid value and iodine value of sunflower oil was investigated by using Sorbaria tomentosa ethanolic extract, synthetic antioxidants (BHA and BHT) at regular intervals of 15 days over storage period of 45 days by following the reported AOAC method [25]
Phytochemical screening of Sorbaria tomentosa extract showed the presence of alkaloids, tannins, flavonoids, carbohydrates, saponins and phenols as major phytochemicals while cardiac glycosides and proteins were present in small amount
Summary
Refined bleached deodorized (RBD) sunflower oil (SFO) was obtained from a local refinery in Lahore. SFO was taken and 250, 500 and 1000 ppm of Sorbaria tomentosa ethanolic extract was added in it. BHA and BHT (200 ppm) were added separately in SFO for comparison. Estimation of Peroxide Value (PV), Free Fatty Acid Value (FFA) and Iodine Value (IV) Peroxide value, free fatty acid value and iodine value of sunflower oil was investigated by using Sorbaria tomentosa ethanolic extract, synthetic antioxidants (BHA and BHT) at regular intervals of 15 days over storage period of 45 days by following the reported AOAC method [25]. Statistical Analysis All analysis were carried out in three replicates and results were expessed as mean ± standard deviations. Significant difference among all readings (p < 0.05) was calculated by applying one way analysis of variance (ANOVA) on statistical package for social sciences (SPSS)
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