Antioxidant Potential and Cytotoxic Effect of Isoflavones Extract from Thai Fermented Soybean (Thua-Nao).

Kanokwan Kulprachakarn,Kongsak Boonyapranai,Nittaya Chansiw,Supakit Chaipoot,Kittipan Rerkasem,Rewat Phongphisutthinant,Wason Parklak,Adchara Prommaban,Banthita Saengsitthisak,Sakaewan Ounjaijean,Kanittha Prakit,Kanjana Pangjit,Narisara Paradee

doi:10.3390/molecules26247432

Abstract

Thua-nao, or Thai fermented soybeans, is a traditional Lanna fermented food in Northern Thailand. It is produced by using a specific bacterial species called Bacillus subtilis var. Thua-nao. We investigated the antioxidant activity and cytotoxic effect of isoflavones from Thua-nao. The phenolic compound contents and total flavonoid contents were determined by spectrophotometry. The antioxidant activity was examined using the ABTS, FRAP, and DPPH assays. The isoflavone contents and phenolic compositions were examined by the high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) techniques. The ability of isoflavones to inhibit human cancer cell growth was assessed by the MTT assay. The total phenolic content, total flavonoid content, and antioxidant activities of the isoflavones were 49.00 ± 0.51 mg GAE/g of dry extract (DE), 10.76 ± 0.82 mg QE/g of DE, 61.03 ± 0.97 µmol Trolox/g of DE, 66.54 ± 3.97 µM FeSO4/g of DE, and 22.47 ± 1.92% of DPPH inhibition, respectively. Additionally, the isoflavone extracts from Thua-nao had high isoflavone contents and polyphenolic compound compositions, especially daidzein and genistein. The isoflavone demonstrated a weak inhibition of MCF-7 and HEK293 cancer cell growth. It has a high antioxidant component, which is beneficial and can be developed for new therapeutic uses. However, further studies on the benefits of Thua-nao should be performed for realizing better and more effective uses soon.

Highlights

Thua-nao is an alkaline fermented food condiment produced from soybeans that are native to Thailand
Thai people liveral health benefits, including being a good source of is protein and containing soy-isoflawhich are well-known for their pharmacological and antioxidant activities
The consumption of soy-fermented foods has been found to provide sevwhich are well-known for their pharmacological and antioxidant activities [11]

Summary

Introduction

Thua-nao is an alkaline fermented food condiment produced from soybeans that are native to Thailand. Thua-nao are a traditional Lanna fermented food in Northern Thailand It is a favorite food consumed among Northern Thai people (i.e., Chiang Rai, Chiang Mai, Mae Hong Son, Lamphun, and Lampang) [1]. It is produced by using a specific bacterial species called. Thua-nao is produced for household consumption, preserved for a longer shelf life, allowing people in the community to have a self-sufficient lifestyle It is often a part of every household for cooking, like shrimp paste in the central region, added to vegetable soup, wrapped in banana leaves, or steamed or grilled enough to eat with sticky rice.

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