Antioxidant potential and chemical characterization of bioactive compounds from a medicinal plant Colebrokea oppositifolia Sm.

Saiqa Ishtiaq,Umer Farooq Awan,Shabnum Shaheen,Mehwish Meo,Iram Liaqat,Uzma Hanif,Muhammad Shuaib,Saraj Bahadur,Wajid Zaman,Memuna Ghafoor Shahid

doi:10.1590/0001-3765202020190387

Abstract

Colebrookea oppositifolia is a highly used medicinal plant and an enriched source of essential oils. Therefore, the present study was designed with the aim to extract the chemical constituents and to evaluate its antioxidant potential. Fresh plant parts were subjected to the extraction of volatile chemical constituents by maceration using n-hexane as the menstruum. The resulting n-hexane fractions were purified and then subjected to GC-MS and FTIR analysis. In-vitro antioxidant abilities were evaluated by, DPPH, total phenolic content (TPC), total flavonoid content (TFC) method against the standard solutions of (Gallic acid, Quercetin) as a positive control. The GC-MS analysis of leaves, stem and inflorescence showed a total of 100, 98 and 48 components out of which 47, 16 and 17 peaks were identified representing the 67.64 %, 73.16 % and 61.93 % of the total oily fractions, respectively. The FTIR spectrum indicated the presence of various functional groups. In-vitro antioxidant results exhibited that leaves showed the highest antioxidant potential by DPPH (3.365 ± 0.002), and the highest total phenolic content by FC method (203.00 ± 0.091). Foliar micromorphological features were found significant in the authentication of C. oppositifolia. Further pharmacognostic studies of this plant are recommended to evaluate its therapeutic potential.

Highlights

Lamiaceae is one of the most important angiosperm family found in most ecosystems of the earth planet (Gul et al 2019a), comprising a variety of members with a distinct aroma
All the solvents n-hexane, methanol were of analytical grade and all chemicals i.e. Quercetin, Gallic-Acid, Butylated Hydroxy Toluene (BHT), sodium phosphate, ammonium Molybdate, aluminum chloride Sodium hydroxide, sodium nitrate, FC reagent were of (Sigma, Germany), Gas chromatographymass spectrometry (GC-MS) analyses were carried out by using Agilent 7890GC/5975MS system Germany
The leaf, stem and inflorescence of C. oppositifolia were subjected to the extraction by maceration of freshly collected leaves in n-hexane and yellowish-green extract (1.37 % w/w)

Summary

Introduction

Lamiaceae is one of the most important angiosperm family found in most ecosystems of the earth planet (Gul et al 2019a), comprising a variety of members with a distinct aroma. It was an ubiquitous plant family in terms of ethnomedicinal properties based on the essential oil concentration produced by trichomes. Essential oils of aromatic plant species were comprehensively employed in pharmaceutical industries owing to their therapeutic effects. Essential oils were adroit health endorsing agents owing to their most important antioxidant attributes (Rehan et al 2014)

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Discussion

Conclusion