Abstract
Supercritical Antisolvent Extraction (SAE) was used in the recovery of antioxidant compounds from mango by-products. The antioxidant extract was obtained by solid–liquid extraction using aqueous acetone (80% v/v) and subsequent adsorption/desorption in C18 cartridge. Recovery of antioxidants was performed by SAE using SC-CO2 as antisolvent. The temperature and pressure parameters were varied in the range 35–45°C and 8–15MPa. The SAE process allowed the recovery of about 90% of the initial phenolic compounds, the best recovery was obtained at 40°C and 10MPa and the main compounds were mangiferin, isomangiferin, quercetin 3-O-galactoside, quercetin 3-O-glucoside, quercetin 3-O-xyloside, quercetin 3-O-arabinoside, quercetin and kaempferol. The product obtained was a dry powder, spherical nanoparticles with a particle diameter of 143±40nm with an antioxidant activity of 851.9μMol TE/g and a half inhibition concentration of DPPH radical of 90μg/mL. The analyses performed indicated that SAE processing of natural extracts is effective for improving the activity of natural antioxidant compounds by producing nanoparticles.
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