Abstract
The present study aimed to evaluate the antioxidant activity of petroleum ether, methanolic extracts and active ingredients separated from Eucalyptus globulus using three different antioxidant assays: 2,2 diphenyl picryl hydrazyl (DPPH), 2,2’- azino-bis [ethylbenzthiazoline-6-sulfonic acid] (ABTS) and β-carotene bleaching assay and identify the mode of action. The results revealed that, crude methanolic extract showed higher antioxidant activity against both DPPH and ABTS radicals than petroleum ether extract. The promising methanol soluble fraction of Eucalyptus globulus wood was fractionated on a silica gel column, using hexane, chloroform and ethyl acetate as the mobile phase to give three fractions (C1, C2 and C3), and both the antioxidant activity and chemical composition for raw and fractions were determined. One of the fractions isolated (C2) showed a remarkable antioxidant activity (EC50 of 64.4 µg/ml, in comparison with 52.74 µg/ml for crude extracts) against ABTS radical method, and the chemical structures of separated active ingredients were identified using different spectroscopic methods such as 17-pentatricontene (C1), N,N-diphenyllauramide (C2) and O-benzyl-N-tert-butoxycarbonyl-D-serine (C3). Also, the mode of action of the promising fraction was determined.
Highlights
Eucalyptus globulus is one of the main forest species in Galicia, representing 27% of total wood volume
The results indicated that, Eucalyptus globulus have high carbohydrate percentage reached to 20% as dry matter and less than lipids and protein content (30.0 and 25.0%) as illustrated in (Figure 1)
Β-sitosterol and stigmasterol have a percentage of 4.11 and 11.14%, respectively. These results are in agreement with those of Gutiérrez et al [21], who studied the chemical composition of lipophilic Extractives from Eucalyptus globulus Labill
Summary
Eucalyptus globulus is one of the main forest species in Galicia, representing 27% of total wood volume. Eucalyptus contains many chemical compounds that play several roles in the plant These include defense against insect and vertebrate herbivores and protection against UV radiation and against cold stress. The synthetic antioxidants include butylated hydroxyanisole and butylated hidroxytoluene (BHA and BHT, respectively), propyl gallate (PG) and tertbutylhydroquinone (TBHQ). Their manufacture costs, the relative poor efficiency of natural tocopherols ( used as antioxidant agents) and the need of increased food additive safety give rise to a crescent demand on other natural and safe antioxidants sources. Extractions of phenolic compounds as antioxidants from eucalyptus (Eucalyptus globulus) bark were done by Vázquez et al [8] who demonstrated the potential of eucalyptus bark as a source of antioxidant compounds. This investigation was designed to identify the mechanism of active ingredients isolated from Eucalyptus globulus for their antioxidant activity, using three different antioxidant methods
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
