Abstract
BackgroundOxidative stress and impaired antioxidant system have been proposed as a potential factors involved in the pathophysiology of diverse disease states, including carcinogenesis. In this study, we explored the lipid peroxidation levels and antioxidant enzyme activities in women diagnosed with different forms of gynecological diseases in order to evaluate the antioxidant status in endometrium of such patients.MethodsEndometrial tissues of gynecological patients with different diagnoses were collected and subjected to assays for superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and lipid hydroperoxides.ResultsSuperoxide dismutase activity was significantly decreased (50% in average) in hyperplastic and adenocarcinoma patients. Activities of both glutathione peroxidase and glutathione reductase were increased 60% and 100% on average, in hyperplastic patients, while in adenocarcinoma patients only glutathione reductase activity was elevated 100%. Catalase activity was significantly decreased in adenocarcinoma patients (47%). Lipid hydroperoxides level was negatively correlated to superoxide dismutase and catalase activities, and positively correlated to glutathione peroxidase and glutathione reductase activities.ConclusionsThis study provided the first comparison of antioxidant status and lipid peroxidation in endometrial tissues of patients with polyps, myoma, hyperplasia and adenocarcinoma. The results showed that patients with premalignant (hyperplastic) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme activities than patients with benign uterine diseases, polyps and myoma, although the extent of disturbance varied with the diagnosis. Further investigation is needed to clarify the mechanisms responsible for the observed alterations and whether lipid hydroperoxide levels and antioxidant enzyme activities in uterus of gynecological patients might be used as additional parameter in clinical evaluation of gynecological disorders.
Highlights
Oxidative stress and impaired antioxidant system have been proposed as a potential factors involved in the pathophysiology of diverse disease states, including carcinogenesis
The results showed that patients with premalignant and malignant lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme activities than patients with benign uterine diseases, polyps and myoma, the extent of disturbance varied with the diagnosis
Our recent results show that gynecological patients have altered AO status in blood, which varies with the enzyme and diagnosis. Both reduction in antioxidants and elevation of lipid peroxidation were observed in general [15]. Since study on this subject and in this area is still rare, we aimed to explore the level of lipid peroxidation and antioxidant enzymes (AOE) activities in endometrium of patients diagnosed with myoma, polyps, hyperplasia simplex, hyperplasia complex and adenocarcinoma in order to evaluate the extent of oxidative stress in endometrial tissue of such patients
Summary
Oxidative stress and impaired antioxidant system have been proposed as a potential factors involved in the pathophysiology of diverse disease states, including carcinogenesis. We explored the lipid peroxidation levels and antioxidant enzyme activities in women diagnosed with different forms of gynecological diseases in order to evaluate the antioxidant status in endometrium of such patients. Studies on the implication of oxidative stress in gynecologic disorders have not received adequate attention, and data regarding mechanisms underlying these diseases are sporadic. Clinical investigations indicate that women with certain common benign gynecological diseases, such as endometriosis and uterine leiomyoma, may have increased risk of developing malignant tumors [5]. Two of the most common malignancies of the female genital tract worldwide are cervical and endometrial cancer [6]
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