Antioxidant enzyme activities and lipid peroxidation as biomarker compounds for potato tuber stored by gamma radiation

Abstract

ObjectiveTo study the capability of gamma irradiation for inhibiting sprouting of potato tubers. MethodsThe enzymes activities i.e. peroxidase (POD), polyphenol oxidase (PPO), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT), in addition to lipid peroxidation level were tested in potato tubers stored for 3, 6 and 9 weeks. Gamma irradiation with five treatments (0, 30, 50, 100 and 200 Gy) was used to control germination process of potato tubers. ResultsGamma radiation was able to maintain potato tuber for 6 weeks. The main biomarkers for validity of potato tuber during storage were studying antioxidant enzyme activities i.e. POD, PPO, GST, SOD, CAT enzyme activities as well as lipid peroxidation during storage time. ConclusionsThe optimum dose was 50 Gy which prevented the sprouting initiation all over the storage period without casting undesirable rotting for potato tubers. At this dose all antioxidant enzyme activities i.e. POD, PPO, GST, SOD, CAT enzyme activities as well as lipid peroxidation level during storage time recorded the best rates.