Abstract
A purified compound with antioxidant properties (28 mg), T3b, was isolated from a methanol extract (10 g) of Origanum majorana L. by sequential procedures, with silica gel column, thin-layer, and LH-20 Sephadex gel column chromatographies. The in vitro scavenging activity of T3b on superoxide anion radical (O 2 − ) was investigated and compared to those of seven commercially available synthetic or natural antioxidants. Of those, the strongest scavenging action was observed in T3b with an IC 50 of 1.44 μg/ml. The T3b also exhibited significant inhibitory effects on 12- O -tetradecanolyphorbol-13-acetate (TPA)-induced O 2 − generation and hydrogen peroxide formation in differentiated HL-60 cells, indicating that the isolated compound is a potent chemopreventer. The purified compound from O. majorana L. was shown to possess both O 2 − scavenging activity and an inhibitory effect against TPA-induced O 2 − generation.
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