Abstract
In the present study, the antioxidant effects of estradiol (E 2) and 2-hydroxyestradiol (2-OHE 2) on microsomal lipid peroxidation induced by Fe 3+/ADP/NADPH and Fe 2+/ascorbate are described. The extent of lipid peroxidation was measured by thiobarbituric acid reactive substances (TBARS) detection, low-level chemiluminescence, and oxygen consumption. 2-OHE 2 had a potent antioxidant activity, which in all cases was higher than that of E 2. In the Fe 2+/ascorbate model, 2-OHE 2 showed a similar pattern of inhibition, irrespective of the presence of NADPH or the functionality of microsomes. However, E 2 produced only a slight inhibition when either denatured microsomes or native microsomes without NADPH were used, whereas it protective effect increased considerably when microsomal E 2 metabolism was favored. During enzymic Fe 3+/ADP/NADPH-induced lipid peroxidation, both E 2 and 2-OHE 2 were found to provide good protection. Results underline the importance of the chemical structure of these compounds and the role of estradiol metabolism in its antioxidant effects. (Steroids 59:383–388, 1994)
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