Abstract
The aim of this study was to determine the effects of bovine serum albumin (BSA) as an antioxidant on post-thaw characters of bull spermatozoa such as motion variables, viability, plasma membrane integrity, morphology, lipid peroxidation, total antioxidant capacity (TAC), total thiols (TT) and the enzymes activities. Ejaculates were collected from six proven bulls and diluted with a citrate-based extender supplemented with various concentrations of BSA (0, 0.5, 1, 1.5 and 2 g/100 mL). The results showed that the semen extender supplemented with various concentrations of BSA increased (p0.05) were detected in progressive motility, sperm track straightness, beat cross frequency, curvilinear velocity and malondialdehyde production between the experimental groups. The highest concentration of BSA (2 g/100 mL) in the semen extender reduced (p<0.05) the sperm motion variables. The results indicated that the most effective concentration of BSA is 1 g/100 mL in the semen extender which is associated with an increase in the total motility, antioxidant enzymes activities, TT and TAC.
Highlights
Artificial insemination with cryopreserved semen has become one of the most effective methods in animal biotechnology and breeding techniques
Addition of bovine serum albumin (BSA) to the freezing extender had a beneficial effect on sperm motility
Addition of 1 g BSA increased (p < 0.05) total antioxidant capacity (TAC) compared to other groups
Summary
Artificial insemination with cryopreserved semen has become one of the most effective methods in animal biotechnology and breeding techniques. Mammalian spermatozoa may damage during semen cryopreservation process (Watson, 2000). It is reported that the cold shock during freeze-thaw process injure mitochondria (Pena et al, 2009), plasma and acrosome membranes (Meyers, 2005) of spermatozoa which may lead to reducing the spermatozoa functional integrity and decreasing its fertilizing aptitude (Gillan & Maxwell, 1999). Free radicals at physiological concentrations are effective in intracellular signaling involved in the regular processes of cell proliferation, differentiation and migration (Piantadosi, 2008). Reactive oxygen species (ROS) are important mediators of normal sperm function, such as signal transduction mechanisms that affect fertility Ashrafi et al / Span J Agric Res (2013) 11(3): 695-701
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