Antioxidant effect of taurine and macroalgae (Sargassum sp. and Gracilaria sp.) extraction on numbers of blood cells and protein profile of mice induced by benzo(α)piren

Abstract

The aims of the study was to elucidate the antioxidant effect of taurine and two macro-algae (Sargassum sp. and Gracilaria sp.) methanol extraction on the number of blood cells (RBCs and leucocytes) and blood protein profile of mice induced by benzo(α)piren. Six experimental groups were applied to this study, they were control group (K-), benzo(α)piren injection only group (K+), benzo(α)piren injection then followed by Sargassum extraction (T1), benzo(α)piren injection then followed by Sargassum extraction and taurine (T2), benzo(α)piren injection then followed by Gracillaria extraction (T3) and benzo(α)piren injection then followed by Gracillaria extraction and taurine (T4). The amount of benzo(α)piren applied by sub-cutaneous injection was 0.3 mg/bw benzo(α)piren for 10 days, while the amount given of Sargassum sp. and Gracillaria sp. extraction each was 8 mg/bw and the amount given of taurine was 15.6 mg/bw. All the extracts and taurine was given to male mice prior benzo(α)piren injection. One way ANOVA was used followed by LSD at a 5% to analyze the collecting data. The results indicated that taurine and both macro-algae were able to normalize back the numbers of red blood cells and leucocytes affected by benzo(α)piren induction. While the blood protein profiles (from the SDS-Page) indicated that there was an increase in expression of 16KDa, expression of protein with 27 KDa (presumably BCl-2 in which its band of 26 KD was phosphorylized) and expression of 41KDa (which presumably α-1-Acid glycoprotein, the protein produced in response to cell damage). Yet the 41 KDa protein was not expressed in groups of both macro-algae combined with taurine.