Antioxidant effect of grapevine leaves extract in rats fed a high fat diet

Abstract

The purpose of this study was to investigate the effect of grapevine leaves extract(GLE) on oxidative stress in rats fed a high fat diet. The 4‐week‐old Sprague‐Dawley rats were divided into six groups; nomal diet group(N), nomal diet +1.0% GLE group(NG1.0), nomal diet +1.5% GLE group(NG1.5), high fat diet group(HF), high fat diet +1.0% GLE group(HFG1.0), high fat diet +1.5 GLE group(HFG1.5). Rats of each group were fed on the corresponding diets for 6 weeks. The thiobarbituric acid reactive substances(TBARS) in high fat and GLE‐treated groups revealed a significant reduction in liver, erythrocytes and plasma compared with the HF group. The erythrocytic activities of catalase(CAT), superoxide dismutase(SOD) and glutathione peroxidase(GSH‐Px) in HFG1.5 group were significantly higer than those of HF group. The hepatic activities of GSH‐Px and glutathione S‐transferase( GST) increased in GLE‐supplemented high fat group compered with HF group. The liver levels of retinol and tocopherol significantly increased in HFG1.5 group compared with those of HF group. Plasma tocopherol level in HFG1.5 group was significantly higher than HF group. These results indicate that grapevine leaves extract exerts a protective effect on high fat diet‐induced oxidative stress in rats. This research was supported by¡°Region‐specific agriculture Development project of RDA(Rural Development Administration), Republic of Korea.