Abstract
The aim of this study was to determine whether a cosmetic formulation elaborated with ozonized theobroma oil may exert beneficial effects in the restoring of the antioxidant activity on the skin of rats previously irradiated with ultraviolet light. 0.5 g of the formulation was applied on the skin of rats for five days. Superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activity were determined in a homogenate of rat skin. Malondialdehyde (MDA), conjugated dienes (CD) and total hydroperoxide (THP) content were determined as biomarkers of oxidative stress. Using these parameters, antioxidant and oxidant activity, redox index and oxidative stress grade were determined. The total antioxidant activity was significantly increased while the redox index, total oxidant activity and oxidative stress grade decreased significantly in damaged rats treated with the formulation. These results show the antioxidant properties of the cosmetic formulation due to the stimulation of antioxidant enzymes such as SOD and GPx, preventing skin injury induced by ultraviolet irradiation.
Highlights
The increasing incidence of disorders in human skin is closely related, among other factors, to solar radiation, mainly because of the unwanted effects induced by ultraviolet radiations contained in the solar spectrum
The development of treatments to avoid oxidative disorders related to aging is becoming more important every day; that is why the aim of this research is to determine the antioxidant activity of a cosmetic formulation elaborated with ozonized theobroma oil in the skin of rats with an oxidative injury
There was a significant increase in the activity of Superoxide dismutase (SOD) in group V compared to the other groups, plus, the activity of this enzyme was inhibited in group III when it is compared with group I
Summary
The increasing incidence of disorders in human skin is closely related, among other factors, to solar radiation, mainly because of the unwanted effects induced by ultraviolet radiations contained in the solar spectrum. When vegetable oils are ozonized a mixture of oxygenated compounds is obtained such as hydrogen peroxide and ozonides which are biologically active (Díaz et al, 2006 a,b) These compounds stimulate the enzymes related to reduction-oxidation processes, which represent an increase in the defensive capacity of the cell against ROS (Menéndez et al, 2008). When these oxygenated molecules come into contact with damaged skin, they slowly decompose to reactive ozone, wich readily dissolves in water, generating hydrogen peroxide and lipid oxidation products that could explain the stimulatory activity of the antioxidant defence system, in addition the skin is oxygenated. When these oxygenated molecules come into contact with damaged skin, they slowly decompose to reactive ozone, wich readily dissolves in water, generating hydrogen peroxide and lipid oxidation products that could explain the stimulatory activity of the antioxidant defence system, in addition the skin is oxygenated. (Bocci, 2005)
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