Abstract
Rice bran is a by-product of rice processing industry, with high levels of phytic acid or phytate. Considering phytic acid antioxidant activity, its various applications and its high concentration in rice bran, this study had the objective of evaluating the antioxidant capacity of purified phytic acid from rice bran using three different methods. Using of 2,4,6-tripyridil-s-triazine or method of FRAP (Ferric Reducing Antioxidant Power) , reducing Fe 2+ activity was not detected for standard or purified phytic acid. With BPS (bathophenanthroline) method, the Fe 2+ chelator activity of standard phytic acid and rice bran phytic acid were dependent on the concentration and contact time and were observed IC 50 values of 4.39 mg mL -1 and IC 50 of 7.54 mg mL -1 , respectively. By the deoxyribose method, the standard phytic acid inhibited the hydroxyl radical with an IC 50 of 0.70 mg mL -1 while the rice bran phytic acid showed a maximum inhibitory activity of 40% associated to its chelating capacity and confirm this important antioxidant capacity.
Highlights
Phytic acid or 1, 2, 3, 4, 5, 6 inositol hexakisphosphate (IP6) is a negatively charged molecule in a wide pH range, with 12 replaceable protons (TSAO et al, 1997)
Considering phytic acid antioxidant activity, its various applications and its high concentration in rice bran, this study had the objective of evaluating the antioxidant capacity of purified phytic acid from rice bran using the Ferric Reducing Antioxidant Power (FRAP), bathophenanthroline disulfonic acidic (BPS), and deoxyribose methods
No reducing capacity of iron ion by standard phytic acid or by purified phytic acid from rice bran using TPTZ was detected, confirming the results of Lee and Hendricks (1995) they observed that adding phytic acid provoked the oxidation of Fe+2 to Fe+3
Summary
Phytic acid or 1, 2, 3, 4, 5, 6 inositol hexakisphosphate (IP6) is a negatively charged molecule in a wide pH range, with 12 replaceable protons (TSAO et al, 1997) These characteristics allow the formation of compounds based on its chelating capacity with polyvalent metals, especially divalent and trivalent cations, making them bio-unavailable (GRAF; EATON, 1990). The iron chelating capacity makes it catalytically inactive, making phytic acid a strong inhibitor of ironmediated hydroxyl radical (OH) production (GRAF; EATON, 1985). It alters the redox potential of iron, keeping it in its ferric form (Fe3+). Considering phytic acid antioxidant activity, its various applications and its high concentration in rice bran, this study had the objective of evaluating the antioxidant capacity of purified phytic acid from rice bran using the FRAP, BPS, and deoxyribose methods
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