Abstract

Boletus edulis is a wild edible mushroom habitually consumed by rural populations. Ethanolic and methanolic extracts was obtained in cold and hot water from dried fruit bodies. The antioxidant activity of freeze-dried extracts from B. edulis were investigated using free radicals scavenging activity, reducing power, metal chelating effect, inhibition of lipid peroxidation, and the identification of antioxidant compounds. The levels of different compounds with antioxidant properties were higher in alcoholic extracts compared with aqueous extracts. Rosmarinic acid was the major phenolic compound, it being identified in a concentration between 7 ± 0.23 and 56 ± 0.15 mg/100 g extract. A positive correlation between the content of total phenols, flavonoids, anthocyanins, and tocopherols, and the antioxidant capacity of the extracts was determined. The results showed that the ethanolic extract of Romanian wild mushroom B. edulis represents a natural source of functional compounds.

Highlights

  • Mushrooms gathered from the wild ora have always been included in the diet of humans for centuries due to their speci c taste as well as their medicinal and curative properties

  • B. edulis extracts have a similar amount of phenolic compounds, as does Agaricus blazei, of approximately 5.8 mg/g. us, it is considered that B. edulis has a higher reducing power than Agaricus bisporus, Coprinus comatus, Pleurotus eryngii, or Pleurotus ostreatus but is lower than Pleurotus citrinopileatus [7]

  • Ethanol and methanol have demonstrated a greater capacity for extraction of biomolecules from B. edulis fruit bodies, which was demonstrated for other species of mushrooms, such as Grifola frondosa [3]

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Summary

Introduction

Mushrooms gathered from the wild ora have always been included in the diet of humans for centuries due to their speci c taste as well as their medicinal and curative properties. Recent researches have demonstrated important medicinal properties by in vitro assays of wild edible mushrooms, which con rm a plethora of established knowledge gleaned from traditional medicine [1]. A current research topic is the determination of these compounds and the effectiveness of some solvents [4]. B. edulis extracts have a similar amount of phenolic compounds, as does Agaricus blazei, of approximately 5.8 mg/g. BioMed Research International e main purpose was to determine the antioxidant capacity of ethanolic, methanolic, and cold water and hot water extracts from the fruiting bodies. E researches included the determination of the reducing power, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, ABTS (2,2 -azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, and the inhibition of hydroxyl radicals, nitric oxide, metal chelating effect, and lipid peroxidation. The quantum of components with antioxidant effect was determined

Materials and Methods
2.11. Determination of Antioxidant Component
2.11.6. Determination of Polyphenol Carboxylic Acids and Flavones
2.11.7. Determination of Tocopherols
Results and Discussion
Conclusions
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