Antioxidant, antiradical power of Syzygium aromaticum essential oil, and its antidermatophytic activity against Epidermophyton floccosum and Trichophyton soudanense

Patience Fankem Mekemzeu Patience Fankem Mekemzeu,Angie Priscille Mantaban Angie Priscille Mantaban,Bienvenue Gnowe Bawane Bienvenue Gnowe Bawane,Samanta Lafortune Mbouwe Chouela Samanta Lafortune Mbouwe Chouela,Manuella Chedjou Guiadem Manuella Chedjou Guiadem,François Nguimatsia François Nguimatsia,Sylvie Kwanga Nguikwie Sylvie Kwanga Nguikwie,Léopold Ngoune Tatsadjieu Léopold Ngoune Tatsadjieu,Pierre Michel Dongmo Jazet Pierre Michel Dongmo Jazet,Victor Moussango Davy Victor Moussango Davy

doi:10.30574/gscbps.2021.15.3.0182

Abstract

In order to provide an effective alternative for efficient management of dermatophytosis, we evaluate in vitro the antioxidant and antiradical potentials of the essential oil of the dry flower buds of Syzygium aromaticum and its antidermatophytic activity against Epidermophyton floccosum and Trichophyton soudanense, For this purpose, the essential oil was obtained by hydrodistillation through a Clevenger apparatus and the antioxidant and antiradical potentials were evaluated by the ferric reducing antioxidant power (FRAP) and the trapping of the ABTS•+ radical methods, respectively. The evaluation of the antidermatophytic activity was made by the agar incorporation method. The results showed that the essential oil reduced ferric iron (Fe3+) to ferrous iron (Fe2+) in a more significant manner than BHT (reference antioxidant). Moreover, the antiradical power of the essential oil was twice greater than that of BHT. Furthermore, the essential oil inhibited the mycelial growth of the two dermatophytes, with 500 ppm and 1000 ppm of minimal inhibitory concentrations against Epidermophyton floccosum and Trichophyton soudanense, respectively. This activity was greater than that of griseofulvin whose minimal inhibitory concentration was greater than 4000 ppm against both studied germs. These findings show that, withon the framework of safeguarding human lives and protecting the environment, the essential oil from the dry flower buds of Syzygium aromaticum appeared as reliable alternative for the treatment of dermatophytosis caused by Epidermophyton floccosum and Trichophyton soudanense. Our results lay scientific foundation toward the promotion and development of Cameroonian biodiversity in treatments of dermatophytosis.

Highlights

Mycosis are infections caused by microscopic fungi, which location can be deep, subcutaneous or superficial [1]
In order to provide an effective alternative for efficient management of dermatophytosis, we evaluate in vitro the antioxidant and antiradical potentials of the essential oil of the dry flower buds of Syzygium aromaticum and its antidermatophytic activity against Epidermophyton floccosum and Trichophyton soudanense, For this purpose, the essential oil was obtained by hydrodistillation through a Clevenger apparatus and the antioxidant and antiradical potentials were evaluated by the ferric reducing antioxidant power (FRAP) and the trapping of the ABTS+ radical methods, respectively
These findings show that, withon the framework of safeguarding human lives and protecting the environment, the essential oil from the dry flower buds of Syzygium aromaticum appeared as reliable alternative for the treatment of dermatophytosis caused by Epidermophyton floccosum and Trichophyton soudanense

Summary

Introduction

Mycosis are infections caused by microscopic fungi, which location can be deep, subcutaneous or superficial [1] When they are superficial, they affect the skin, integuments (nails, body hair and hair) and mucous membranes. Dermatophytes are a group of filamentous fungi able of digesting keratin and responsible of dermatophytosis [4] They affect 20 % to 25 % of the world's population [5]. The management of dermatophytosis in modern medicine requires antifungals drugs such as terbinafine, itraconazole, ketoconazole and griseofulvin [8]. The latter effective, present risks of toxicity, undesirable side effects and are sometimes inaccessible for low income people [9]. That’s why our interest in this study was to evaluate the antioxidant, antiradical and antidermatophytic activities of the dry flower buds of Syzygium aromaticum and contribute in the research of alternative treatments for dermatophytosis

Methods

Results

Conclusion