Antioxidant, Antimicrobial and Phenolic Component Analysis of Some Edge Medicinal Plants

Abstract

Objective: The plants, which grow wild in the Eastern Anatolia Region and are known as kari (Arum elongatum;Ae) and mende (Chaerophyllum macrospermum;Cm), are used for treatment purposes such as pain reliever and wound healing properties. It is aimed to determine some biological properties of these plants, which are also used medicinally.
 Material-Method: Firstly, hexane(H), ethanol(E) and acetone(A) extracts of dried plants were prepared and antimicrobial activities of extracts against 13 indicator microorganisms were determined by agar well diffusion method. Antioxidant activities were determined by DPPH and ABTS analyses and the total phenolic component contents were determined by the Folin-Ciocalteu method. 
 Results: The maximum extract yield was obtained in the ethanol extracts of the plants (AeE;10.4011% CmE;2.4898%), the minimum extract yield was obtained in the acetone extracts (AeA;0.8422% CmA;0.3510%). The hexane extract of A. elongatum has no inhibition activity against E. faecalis and L. monocytogenes, but it has inhibition activity against other indicator microorganisms. The other extracts of A. elongatum and all extracts of C. macrospermum were found to have inhibitory activity against all indicator microorganisms. Furthermore, the highest phenolic content was calculated as 54.60±0.486 µg mL-1 (AeE) and the lowest content as 3.47±0.001 µg mL-1 (CmA). As a result of the DPPH method the highest IC50 was calculated as 32.4140 µg mL-1 (CmH) and the lowest IC50 as 12.7876 µg mL-1 (AeA) and ABTS method the highest IC50 was calculated as 10.3203 µg mL-1 (CmE), and the lowest IC50 as 0.2744 µg mL-1 (CmA). 
 Conclusion: These results show that the plant extracts have strong antimicrobial activity and antioxidant activity.