Antioxidant, antimicrobial and antiproliferative activities of fungal metabolite produced by Aspergillus flavus on in vitro study

Taghreed N Almanaa,Nashwa El-Gazzar,Noha Saleh,Gamal Rabie,Rasha M El-Mekkawy,Marwa A Yassin

doi:10.1590/fst.01421

Abstract

Regarding to the defiance came upon scientists to output natural products with potent biological activity from cheap sources and in an attempt to recover the average of antiproliferative agents, the employment of fungal metabolite in creating a cordial proper process was a prerequisite. In the present results, fungal metabolites were detected by thin layer chromatography. One fungal isolate out of ten isolates bioassayed, was showed to have the most potent antioxidant, antiproliferative and antimicrobial activity; this fungal isolate was identified as belonging to Aspergillus flavus (A. flavus). Fungal extract of A. flavus showed a high free radical scavenging activity using Diphenyl-1-picrylhydrazyl (DPPH) scavenging % with low a concentration of IC50 and potent flavonoid content. The result of instrumental analysis using GC.Mass illustrates the presence of different potent products of A. flavus extract. In addition, A. flavus extract showed a promising antiproliferative activity. Inhibitory activity against Hepatocellular carcinoma cells was detected under these experimental conditions. This strategy can be further used to elicit new age drugs.

Highlights

For a wide variety of secondary metabolites, fungi are the most diverse, where different primary extracts play a powerful role in the biological activity of species (Ronsberg et al, 2013; El-Gazzar & Enan, 2020)
The antioxidant activity of the extracellular extracts are higher than the intracellular extracts of the fungal isolates
The biological efficacy of certain secondary metabolites as antioxidant, antimicrobial and antitumor was suggested for the filamentous fungi; this finding is in accordance with the observations of Chen et al (2011); El-Gazzar et al, (2021); El-Bahr et al (2021)

Summary

Introduction

The existence of numerous issues of cancer disease and antibiotic resistant strains is increased recently and their inhibition by alternative agents are necessary (Shi et al, 2007; El-Gazzar et al, 2020; Abdel-Shafi et al, 2020; Enan et al, 2020; El-Sayed et al, 2020). The biocontrol of this cancer is of interest to persist study to detect and discover newly solution to prevent such disease in vitro (Shi et al, 2007) In this regard, there is an inevitable and urgent medical need for natural bioactive metabolites with novel anticancer and antimicrobial activity (Abdel-Shafi et al, 2020; Enan et al, 2018) and there is frequently concern in detection protocols to create confident and appreciate-efficient biocidal products (El-Gazzar et al, 2020; El-Gazzar & Ismail., 2020). Recent perspectives all over the world are to use safe materials as either safe therapy or as a biosorptive tools for anticancer and antimicrobial activity (Enan et al, 2020; Abdel‐Shafi et al, 2020; Osman et al, 2021) In this framework, natural metabolites from fungi are used to create confident and appreciate-efficient biocidal products by applying the material technology, industrial processes and others (El-Gazzar et al, 2020). Derivative extracts from fungal strains are distinguished by incoming inherent outputs that are subject to characterize new medicine and industries (Suryanarayanan et al, 2009)

Methods

Results

Discussion

Conclusion