Antioxidant, antimicrobial and anticholinesterase activity of Dittrichia graveolens

Abstract

Plants are responsible for many different biological activities. In our study, antioxidant, antimicrobial and anticholinestrase activities of Dittrichia graveolens (L.) Greuter were determined. In addition, the total phenolic and flavonoid contents of the plant were measured. The aerial parts of the plant were extracted with ethanol. Antioxidant activities of the extracts were measured with Rel assay kits. Antimicrobial activity was determined using the agar dilution method. Anticholinesterase activity was determined by measuring acetyl and butyrylcholinesterase activities. Determination of total phenolic content was determined using the Folin-Ciocalteu reagent. Total flavonoid quantification was performed using aluminum chloride assay. As a result of the study, the TAS value of the plant was determined as 6.933±0.121, the TOS value was 12.535±0.244 and the OSI value was 0.181±0.006. It was determined that the plant extract was effective against microorganisms at concentrations of 50-400 µg/mL. Additionally, the anti-AChE value was found to be 25.88±1.73 and the anti-BChE value was 45.32±2.26. Total phenolic content was determined as 86.42±2.72, and total flavonoid content was determined as 117.96±1.93. As a result, it was determined that D. graveolens has antioxidant, antimicrobial and anticholinesterase activity.