Abstract
Introduction and Aim: Plants are considered to be novel source of active compounds having pharmacological properties and help in the development of therapeutic agents. Hence, this study was undertaken to evaluate the antioxidant, anti-inflammatory, analgesic and anti-proliferating activity of aqueous and methanolic leaf extracts of Grewia heterotricha Mast.
 Materials and Methods: The aqueous and methanolic leaf extracts of the plant were assessed for their in-vitro antioxidant activity by DPPH radical scavenging activity, in-vivo anti-inflammatory activity by carrageenan induced rat paw edema method, in-vivo analgesic activity by acetic acid-induced writhing test and in-vitro anti- proliferating activity by MTT assay.
 Results: The methanolic extract had shown very significant DPPH radical scavenging activity with IC50 value 98.95?g/ml than aqueous extract and showed a significant reduction in the paw volume of rats at the concentration of 100 mg/kg body weight indicating potent anti-inflammatory activity compared with the reference standard Diclofenac sodium. Both the extracts showed significant analgesic effect (p<0.001) in acetic acid-induced pain models in a dose dependent manner. The methanolic extract showed higher analgesic activity compared to aqueous extract by inhibiting the pain indicated by a decrease in the number of writhes. In addition, both the extracts showed a decrease in MCF-7 cell viability at the concentration of 550µg/ml. Compared to the aqueous extract, MEGH has shown more cytotoxic effect on the cancer cell lines.
 Conclusion: The results suggest that both aqueous and methanolic extracts of Grewia heterotricha Mast. leaves possess potent antioxidant, analgesic, anti-inflammatory and anti-proliferating properties, which supports the use of the plant in traditional medicine. Further investigation is required to illuminate on its active compounds.
 
 Keywords: Analgesic; anti-inflammatory; DPPH; cytotoxic.
Highlights
Introduction and AimPlants are considered to be novel source of active compounds having pharmacological properties and help in the development of therapeutic agents
Synthetic drugs like non-steroidal antiinflammatory drugs (NSAIDS) and corticosteroids are used in the treatment of inflammations
Anti-inflammatory activity was evaluated by carrageenan-induced paw edema model inet Wistar rats
Summary
Introduction and AimPlants are considered to be novel source of active compounds having pharmacological properties and help in the development of therapeutic agents. Tissue damage provokes an inflammatory response by the production of mediators and chemotactic factors [2]. An uncontrolled and persistent inflammation may lead to chronic diseases like asthma, rheumatoid arthritis or cancer [3]. Synthetic drugs like non-steroidal antiinflammatory drugs (NSAIDS) and corticosteroids are used in the treatment of inflammations. Even though these drugs are effective, they produce severe side effects such as peptic ulceration, osteoporosis and GIT bleeding [4, 5]. Cancer is one of the major causes of mortality in developed and developing countries and its severity is only to cardiac diseases. There is a need for new therapies and drugs to treat and prevent life-threatening diseases [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
