Antioxidant, Anti-inflammatory and Anthelminthic Activities of Traditionally used Ocimum basilicum L., and Ocimum tenuiflorum L. Extract Loaded Emulsion

Abstract

The demand for cosmeceuticals is rapidly expanding. Various phytoconstituents are identified from Ocimum basilicum and O. tenuiflorum and have several pharmacological properties. The present study aimed to formulate a novel alternative herbal formulation for topical administration with natural antioxidant properties for the treatment of inflammatory conditions with the destructive capacity of parasitic worms. The egg albumin denaturation technique was used to evaluate the test formulations’ and the standard’s in-vitro anti-inflammatory properties, and the hydrogen peroxide assay was used to measure the extracts’ antioxidant properties. Eudrilus eugeniae was the target of the investigation into in-vitro anthelmintic action. The pH of the cream was 6.8 (average), it was easily spreadable and washable, no gritty particles were found and the dye test confirmed that it was an o/w type of cream. The egg albumin denaturation technique was utilized to evaluate the anti-inflammatory properties. The results cleared those herbal formulations (F1 and F2) and protein denaturation was decreased by diclofenac sodium (Standard) in a dose-dependent manner. Test samples exhibited higher anti-inflammatory activities than the standard with their percentage inhibitions being 41.5 ± 0.5 (F2), 39.1 ± 0.4 mg/mL (F1), and 37.3 ± 0.3 mg/mL (F-Standard), respectively. Antioxidants are compounds that either completely stop or significantly reduce the harm that free unstable radicals. F2 showed a higher concentration-dependent anthelmintic activity than F1 and standard albendazole. Increasing the concentration decreases the paralysis and death time of earthworms. Finally concluded that F2 indicates higher anti-inflammatory and anthelmintic activities than others with high natural antioxidant power.