Abstract
The East Indian sandalwood oil has been widely used as an Ayurvedic medicine and has multiple pharmacological properties. However, antioxidant and antistress potency of East Indian sandalwood oil against oxidative stress-induced damages remain unexplored. Thus, the present study was aimed to investigate the in vitro and in vivo antioxidant and stress-protective properties of essential oil extracted from the heartwood of plantation-grown Santalum album L. (SEO). The SEO was extracted by traditional stream distillation method and the chemical components were determined via gas chromatography-mass spectrometry (GC–MS) analysis. Nineteen chemical compounds were identified by GC–MS, accounting for 96.81% of total oil. The main components were α-santalol (41.77%), β-santalol (18.02%), (Z)-α-trans-bergamotol (8.50%), (Z)-lanceol (6.57%), and epi-β-santalol (5.78%). The in vitro results suggests that SEO possess an excellent antioxidant activity as it was able to inhibits the intracellular reactive oxygen species (ROS) generation, reverse the morphological damages and effectively improves viability of neural cells under oxidative stress conditions. Moreover, SEO markedly increased the antioxidant enzyme activities of superoxide dismutase, catalase, and glutathione peroxidase. Western blotting results further confirmed that SEO protects neural cells against oxidative damages possibly by activating the nuclear factor-erythroid 2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) antioxidant mechanism. The SEO also exhibited a strong in vivo antioxidant and stress modulatory activity on Caenorhabditis elegans. The SEO not only acts protective against oxidative stress but also prolongs the lifespan of mev-1 mutant C. elegans having shortened lifespan due to the over production of ROS. This data confirmed that SEO exert potent antioxidant and stress modulatory activities possibly by direct scavenging of free radicals and activation of antioxidant defense system, in vitro and in vivo. Together, these findings indicated that SEO has the potency to be utilized as a source of antioxidant for treating several degenerative and disease conditions caused by oxidative stress.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have