Abstract
The antioxidant and hepatoprotective activities of ethyl acetate extract was carefully investigated by the methods of DPPH radical scavenging activity, Hydroxyl radical scavenging activity, Superoxide radical scavenging activity, Hydrogen peroxide radical scavenging activity and its Reducing power ability. All these in vitro antioxidant activities were concentration dependent which were compared with standard antioxidants such as BHT, α-tocopherol. The hepatoprotective potential of Podophyllum hexandrum extract was also evaluated in male Wistar rats against carbon tetrachloride (CCl4)-induced liver damage. Pre-treated rats were given ethyl acetate extract at 20, 30 and 50 mg/kg dose prior to CCl4 administration (1 ml/kg, 1:1 in olive oil). Rats pre-treated with Podophyllum hexandrum extract remarkably prevented the elevation of serum AST, ALT, LDH and liver lipid peroxides in CCl4-treated rats. Hepatic glutathione levels were significantly increased by the treatment with the extract in all the experimental groups. The extract at the tested doses also restored the levels of liver homogenate enzymes (glutathione peroxidase, glutathione reductase, superoxide dismutase and glutathione-S- transferase) significantly. This study suggests that ethyl acetate extract of P. hexandrum has a liver protective effect against CCl4-induced hepatotoxicity and possess in vitro antioxidant activities.
Highlights
The partially reduced metabolites of oxygen and nitrogen, commonly referred to as free radicals are highly toxic and reactive
The antioxidant activity of the ethyl acetate extract of the rhizome of P. hexandrum was investigated by using DPPH scavenging assay, hydroxyl assay, H2O2 assay, superoxide assay, and reducing power
Our results suggest that polyphenolic components within the ethyl acetate extract of P. hexandrum play an important role in scavenging of free radicals, and the scavenging activity is increased with the increasing concentration of the plantextract
Summary
The partially reduced metabolites of oxygen and nitrogen, commonly referred to as free radicals are highly toxic and reactive. They have been postulated to be increased in majority of diseases like aging, atherosclerosis, cancer, diabetes, liver cirrhosis, cardiovascular disorders, and so forth, [1, 2]. The liver, because of its strategic anatomical action and its large capacity for metabolic conversions, is exposed to many kinds of xenobiotics and therapeutic agents Due to these facts, efforts to find suitable curatative agents for treatment of liver diseases in natural products of plant and mineral origin are being made [2]. Liver injury induced by CCl4 is the best characterized system of xenobiotics-induced hepatotoxicity in living system It is a volatile organic chemical and causes liver, kidney and lung damage through free radical mediated process
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