Abstract
The hot-water extract of seaweed Sargassum hemiphyllum was evaluated antioxidant activity by using four different methods, including DPPH free radicals scavenging activity, Fe(superscript +2) chelating activity, superoxide anion radical scavenging activity and reducing power, and the immune-stimulating activity by using HB4C5 cells and J774.1 cells. It was found that the antioxidant activity was increasing in correlating with the concentration below 3.5 mg/ml. The HB4C5 cells showed the maximum relative activities of cell proliferation (174%) and IgM secretion (132%) at 120 μg/ml of hot-water extract. Overheating slightly reduced the immune-stimulating activity of hot-water extract. At 80 μg/ml, J774.1 cells showed the maximum relative activities of cell proliferation (141%) and phagocytosis (147%). Hence, the hot-water extract of seaweed S. hemiphyllum plays the important role on antioxidant and immune-stimulating activities.
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