Antioxidant and hypoglycemic effects of Justicia spicigera in kidney from diabetic rats

Abstract

Diabetes mellitus is a metabolic disease characterized by hyperglycemia, with alterations in the metabolism of carbohydrates, lipids and proteins, where there is an absolute or relative deficiency in the secretion and/or action of insulin, or both. Currently, the complications of DM with oxidative stress have been related, causing oxidative damage to biomolecules that can not be counteracted by the defense antioxidant systems, since they are diminished. Such oxidative damage can be counteracted with the administration of antioxidants such as those present in the leaves of Justicia spicigera. Solvents of different polarity were used: Hexane, ethyl acetate and ethanol, since each solvent carries different types of compounds and were tested in vitro antioxidants of: Antilipoperoxidation, reducing power, reduction of phosphomolybdenum and reduction of DPPH radical. In the reduction of the radical DPPH, the hexane and ethanolic extracts equalized the reduction of ascorbic acid with 100% and the ethyl acetate extract 70%. For the reduction of phosphomolybdenum, the hexane extract reduced it by 28%, the extract of ethyl acetate by 94%, and the extract ethanolic by 100%. The reduction of Fe3+ in the hexane extract was 9%; 17% ethyl acetate extract and the ethanolic extract reduced it by 25%. In the antilipoperoxidation technique, the hexanic and ethanolic extract inhibited lipoperoxidation by 82 and 79% respectively, while the extract of ethyl acetate by 53%. For the in vivo tests, male Wistar rats were used to obtain the kidneys. The GSH/GSSG ratio of the kidney mitochondria, the diabetic control group and the hexane and ethyl acetate extracts was of 0.8, unlike the ethanolic extract that significantly improved it to 2.0. Since in DM there is alteration in lipid and protein metabolism, it was of interest to analyze cholesterol, triglycerides, urea and serum creatinine. In the serological tests, the hexanic extract presented only hypolipidemic effect in the diabetic group. Ethyl acetate and ethanolic extracts had hypoglycemic and hypotriglyceridemic effects in diabetic rats. In conclusión, the hexane extracts, ethyl acetate and ethanol from the leaves of Justicia, are of beneficial effects of a serological and antioxidant level.Support or Funding InformationThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.