Abstract
BackgroundLawsonia inermis (Lythraceae) is an ethnomedicinal plant, traditionally known for curing several ailments such as skin diseases, bacterial infections, jaundice, renal lithiases and inflammation etc. The present work deals with assessment of in vitro antioxidant and in vivo hepatoprotective potential of butanolic fraction (But-LI) of Lawsonia inermis L. leaves.MethodsAntioxidant activity was evaluated using deoxyribose degradation, lipid peroxidation inhibition and ferric reducing antioxidant power (FRAP) assay. In vivo protective potential of But-LI was assessed at 3 doses [100, 200 & 400 mg/kg body weight (bw)] against 2-acetylaminofluorene (2-AAF) induced hepatic damage in male Wistar rats.ResultsBut-LI effectively scavenged hydroxyl radicals in deoxyribose degradation assay (IC50 149.12 μg/ml). Fraction also inhibited lipid peroxidation and demonstrated appreciable reducing potential in FRAP assay. Treatment of animals with 2-AAF resulted in increased hepatic parameters such as SGOT (2.22 fold), SGPT (1.72 fold), ALP (5.68 fold) and lipid peroxidation (2.94 fold). Different concentration of But-LI demonstrated pronounced protective effects via decreasing levels of SGOT, SGPT, ALP and lipid peroxidation altered by 2-AAF treatment. But-LI administration also restored the normal liver architecture as evident from histopathological studies.ConclusionsThe present experimental findings revealed that phytoconstituents of Lawsonia inermis L. possess potential to effectively protect rats from the 2-AAF induced hepatic damage in vivo possibly by inhibition of reactive oxygen species and lipid peroxidation.
Highlights
Lawsonia inermis (Lythraceae) is an ethnomedicinal plant, traditionally known for curing several ailments such as skin diseases, bacterial infections, jaundice, renal lithiases and inflammation etc
In our previous reports [51, 52], we reported extract/fractions of L. inermis with antioxidant, antiproliferative and apoptosis inducing activity
IC50 of 149.12 μg/ml was obtained from regression equation showed that fraction was more potent than standard rutin (IC50 203.56 μg/ml)
Summary
Lawsonia inermis (Lythraceae) is an ethnomedicinal plant, traditionally known for curing several ailments such as skin diseases, bacterial infections, jaundice, renal lithiases and inflammation etc. Mutations resulting spontaneously or from environmental exposure may lead to cancer [1]. Existence of DNA depends upon formation and breaking of bonds. It is not astonishing that DNA regularly endures various kinds of chemical damages due to spontaneous thermal effects and as result of attack of other reactive molecules [2]. Other example was bladder carcinogenesis incidence among the workers working in the rubber and chemical industries. This led to the recognition of 2-naphthylamine as bladder carcinogen [2]. With advancement in the science, it is well known that some of cancers are environmental in origin and can be related directly to different chemical exposures [7]. Humans are constantly exposed to plethora of xenobiotic chemicals and other related environmental pollutants which are hazardous to the health [8]
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