Antioxidant and enzyme-inhibiting activity of lyophilized extract from Clinopodium vulgare L. (Lamiaceae)

Gazela Nassar-Eddin,Nikolay Danchev,Rumyana Vitanska-Simeonova,Dimitrina Zheleva-Dimitrova

doi:10.3897/pharmacia.68.e61911

Abstract

Clinopodium vulgare L. (Lamiaceae) was used in the traditional Bulgarian medicine for treatment of wounds, diabetes and gastric ulcers. The aim of the present study was to evaluate the antioxidant capacity of the extract (CVE) and fractions from C. vulgare (CV) using DPPH, ABTS and FRAP methods. Enzyme inhibitory activity against acetylcholinesterase, α-glucosidase and α-amylase was also investigated. Rosmarinic acid was used as a positive control. The fraction CV3 demonstrated the highest radical scavenging activity with IC50 values of 0.02 mg/ml (DPPH) and 0.0002 mg/ml (ABTS), as well as the strongest ferric reducing potential (FRAP) of 0.89 mM TE/mg dw. The crude aqueous-methanol extract of C. vulgare also showed high activity with IC50 values of 0.05 mg/ml (DPPH), 0.04 mg/ml (ABTS) and 0.89 mM TE/mg dw (FRAP). Moreover, CV3 demonstrated moderate α-glucosidase and α-amylase inhibitory potential.

Highlights

Clinopodium vulgare L. (Lamiaceae) is a perennial herbaceous plant widespread in Bulgaria
Various antioxidant activity tests were performed to determine the antioxidant profile of the crude plant extract C. vulgare extracts (CVE) and 3 different fractions
The crude CVE showed high activity with IC50 of 0.05 mg/ ml, 0.04 mg/ ml and 0.89 mM Trolox equivalent (TE)/mg dw for DPPH, ABTS, and FRAP respectively

Summary

Introduction

Clinopodium vulgare L. (Lamiaceae) is a perennial herbaceous plant widespread in Bulgaria. The genus Clinopodium L. consist of flowering plants, widely distributed in southern and southeastern Europe, North America, Latin America and Asia (Saltos et al 2014). Previous investigations revealed a variety of beneficial effects of C. vulgare extracts (CVE) i.e. anti-bacterial, anti-inflammatory, antioxidant and anticancer activities (Tepe et al 2007; Burk et al 2009; Stefanovic et al 2011; Batsalova et al 2017). It was established the acute and subacute toxicity of C. vulgare lyophilized water extract (Zheleva-Dimitrova et al 2019)

Objectives

Methods

Results