Antioxidant and cytotoxic activity of diplotropis incexis rizzini & a. Mattos seed oil in b16-f10 murine melanoma / Actividade antioxidantes e citóxicos de diplotropis incexis rizzini & a. Mattos semente de petróleo em b16-f10 murina melanoma

Abstract

Introduction: Diplotropis incexis is popularly known as sucupira. To extract its oil, which is used for the treatment of eczema, inflammation, skin patches, rheumatism, and to treat hemorrhages, it is necessary to breakdown and decoct the seeds. The aim was to evaluate the antioxidant activity and cytotoxic effect of Diplotropis incexis oil (DIO) in B16-F10 murine melanoma cells. Materials and methods: DIO was obtained from the breakdown of seeds, Soxhlet extraction, and rotary evaporation. Antioxidant activity was measured by radical scavenging. Quantification of phenolic groups was performed using a standard curve of gallic acid and quercetin. DIO toxicity was determined between 0.01 – 100 µg/mL by photometry (MTT) and cytometry. Finally, cell morphology was examined by confocal microscopy. Results: The DIO showed antioxidant potential, being about 335x less potent than the gallic acid control. 109 mg of total phenols and 381 mg of flavonoids were found for every 100g of crude oil. The DIO showed high cytotoxicity against the B16-F10 line, presenting IC50 = 10 µg/mL for cells treated for 48 hours. The cells underwent drastic morphological changes, presenting changes in membrane structure and drastic changes in the cytoskeleton, due to F-actin filament disarrangement. Conclusions: The first reports of DIO activity showed that it has moderate antioxidant activity with the presence of phenolic groups in its composition. In addition to having cytotoxic activity in the B16-F10 cell lines, it is able to promote decreased cell viability and morphological changes depending on concentration and time spent in contact with oil.