Abstract
Background: Terminalia arjuna, a popular cardio curative plant. Present work emphasizes on the evaluation of antioxidant and anti-urease activity of different fractions extracts of Terminalia arjuna seeds. Methods: Six alternate fractions including methanol, ethanol, n- butanol, ethyl acetate, dichloromethane and distilled water were separately used for extraction of Arjun seeds. Antioxidant activity was evaluated through free radical scavenging (DPPH) assay method, using 1, 1-diphenyl-2- picryl hydrazyl (DPPH) as a standard free radical. Anti- urease activity was also assessed through anti-urease absorbance assay method. Results: Methanol and ethanol extracts showed highly significant results (88±1.52 and 87±0.057 % inhibition respectively) than other fractions extracts but less significant than the standard ascorbic acid (93.73 ±1.12). All various fractions extracts of Arjun seed exhibited anti-urease activity up to some extent, but ethyl acetate extract exhibited highest results than other extracts as 70.4±0.15% inhibition of urease followed by n-butanol, ethanol, dichloromethane, methanol and aqueous extract respectively. Conclusion: Present work is a sort of novel work as these activities of Arjun seed have not been reported till now. Evidence of the current study have provided a new way to utilize anti-urease and antioxidant potential of Arjun seeds for therapeutic purpose in current of various diseases.
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