Abstract
Buckwheat is known not only due to its appropriate nutritional composition but the content of prophylactic compounds, too. These are responsible for buckwheat beneficial impact on human health. Most of them are concentrated in outer layers of buckwheat grain. The subject of this work was to screen hulls of nine common and one tartary buckwheat cultivar for the content of flavonoids and its antioxidant and antiproteinase effects. The highest content of total flavonoids was determined for tartary buckwheat cultivar Madawaska (0.6% of hulls weight). Among common buckwheat cultivars the best values reached samples Bamby (0.23%) and KASHO-2 (0.11%). Antioxidant activity as detected via binding radical ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) and monitoring reducing power was the most effective for samples with highest flavonoid content. Buckwheat hulls effectively inhibited pathophysiological proteases thrombin and urokinase, whereas only little effects were seen to trypsin and elastase. In this testing there were again the best samples with highest flavonoid content. Only tartary buckwheat Madawaska effectively inhibited elastase at tested concentrations. No significant correlation was determined between flavonoid content and measured antioxidant or protease inhibitory action. Obtained results allow us to commend tartary buckwheat cultivar Madawaska as well as common buckwheat cultivars Bamby and KASHO-2 for further experiments.
Highlights
Food is a source of energy and nutrition for maintenance and growth of the body but is a source of bioactive compounds that have beneficial effects on humans
Because flavonoids are known for their antioxidant properties and our previous investigations have shown, that buckwheat hull extracts possess antioxidant action, in the step we have examined their antioxidant activity using other two different spectrophotometric methods
Antioxidant activity determined via binding radical ABTS as well as measuring reducing power (FRAP) was the highest in the case of tartary buckwheat Madawaska, which concurrent contained the highest amount of total flavonoids among tested samples
Summary
Food is a source of energy and nutrition for maintenance and growth of the body but is a source of bioactive compounds that have beneficial effects on humans. Inhibitory activity to serine proteases trypsin, thrombin, urokinase and elastase was determined. A.) for 24 hours at room temperature (diluent : weighing material = 10 : 1), filtered and used for flavonoid content determination and antioxidant activity testing. In comparison with other parts of buckwheat grain in hulls are concentrated present polyphenols and flavonoids (Sedej et al, 2012).
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