Abstract
Moringa oleifera Lam. is a highly valued medicinal plant in India, especially Kerala. In the present study, antioxidant activity of aqueous extract of leaves of M. oleifera was determined both in-vitro and in-vivo. Male Wistar rats of 3 age groups- 6, 12, and 18 months old were used for in-vivo analysis. In vitro anti-proliferative effect of the extract was carried out in Dalton’s Lymphoma Ascites (DLA) Cells. LCMS-QTOF analysis of the extract was also done to determine the bioactive components present in the extract. Antioxidant activity of M. oleifera leaf showed an IC 50 value of 10.47 ?g/ml and whereas for standard drug, ascorbic acid, it was 19.52 ?g/ml. In-vivo analysis of lipid peroxidation showed a significant reduction of lipid peroxidation in the brains of 12 and 18-months old treated groups. Up to 75% mortality of DLA cancerous cells was observed in-vitro in different concentrations of M. oleifera leaf water extract in a dose-dependent manner, demonstrating its anti-proliferative property. LCMS-QTOF analysis revealed the presence of emodin-8-glucoside in the extract. Molecular docking analysis (Auto Dock Vina) of emodin-8-glucoside with six cancer related proteins showed highest binding affinity with AKT-1 with a binding score of -10.4 kcal/mol, also showed good affinity with NF-kB (p65), Stat-3, Bcl-2, Bcl-xl and c-FLIP. This study helps to choose healthy diet practices to overcome free radical onslaught and cancerous cell proliferation especially in the later stages of life. This can also pave way for the emergence of diet based therapeutic cure for cancer.
Highlights
Studies of pharmacological applications of medicinal plants used in the diet entails a new arena of research
Moringa oleifera Lam. (Family Moringaceae), is called as horseradish tree, drumstick tree in English and in Sanskrit, it is called as Shigru
Percent yield for lyophilized aqueous extract of M. oleifera leaf was 30% (Table 2)
Summary
Studies of pharmacological applications of medicinal plants used in the diet entails a new arena of research. There is need for research to understand the nontoxicity and economic viability of these medicinal plants being used for various pharmacological interventions, especially to fight against cancer [1]. (Family Moringaceae), is called as horseradish tree, drumstick tree in English and in Sanskrit, it is called as Shigru. It has been an ingredient of our diet for several centuries. Bioactive compounds present in M. oleifera include vitamins, carotenoids, polyphenols, flavonoids, essential amino acids and phenolic acids. All the parts of the tree are edible and possesses various pharmacological actions and used in the Indian traditional medicine to cure to various ailments; and is called ‘miracle tree’ [79]
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