Antioxidant and antimutagenic activities of Viscum album fruit ethanolic extract in human lymphocytes

Abstract

Polyphenolic compounds are widely distributed in plants and known to be excellent antioxidants in vitro. They have the capacity to reduce free-radical formation by scavenging free-radicals. In this study we have evaluated the antioxidant and antimutagenic potencies of polyphenolic compounds of Viscum album against trichloroethylene (TCE)-induced oxidative and genotoxic damage. V. album extract (VAE 0.5 μg/ml) protected human lymphocytes against TCE. In chromosomal aberration (CA) analysis, no significant increase in total aberrations were found after treatment with TCE and all VAE concentrations. The mitotic index (MI) showed significant increase in 0.5 µg/ml VAE samples when compared with TCE-treated (2 µM) group. VAE (0.5 µg/ml) reduced the levels of malondialdehyde (MDA) significantly wherease VAE (1.0 and 2.0 µg/ml) samples increased MDA concentrations significantly. We have also shown that the various DNA effects of TCE treatment seem to be DNA damages, but not mutations as TCE treated profiles were reverted back to the control like profiles by most probably DNA repair mechanisms in VAE 0.5 μg/ml treated group. Key words: Viscum album, polyphenolic compounds, antioxidant, DNA instability, lipid peroxidation.