Antioxidant and antimicrobial extracts obtained from agricultural by-products: Strategies for a sustainable recovery and future perspectives

Abstract

Durum wheat and rice brans are by-products deriving from the milling industry and source of important phytochemicals, mostly phenolics in bound form. The objectives of this work were to evaluate the most effective method for the extraction of phenolic acids from these by-products with antioxidant and antimicrobial activity and to standardize the obtained extracts to encourage their commercialization in health field. Free phenolics were extracted with ultrasound-assisted extraction (UAE) and bound phenolics with alkaline hydrolysis (AH) and ultrasound-assisted alkaline hydrolysis (UAAH). Extracts were analyzed with HPTLC (high-performance thin-layer chromatography) and RPHPLC-DAD-ESIMS (reversed-phase high-performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry); the content of trans-ferulic acid, p-coumaric acid, total phenolics were also quantified. The antioxidant activity was investigated using DPPH and ABTS methods. The antimicrobial activity was evaluated against Staphylococcus aureus and Staphylococcus epidermidis strains. Results obtained using UAAH showed the most interesting data, suggesting this method as the most effective to obtain active extracts from the by-products. Durum wheat, extracted with UAAH, exhibited the highest content of trans-ferulic acid (406.14 ± 0.65 μg FA/mg extract), total phenolics (610.58 ± 57.60 mg GAE/g dried extract) and good antioxidant and antimicrobial activities and it has been selected to formulate oil-in-water cosmetic products.