Abstract
Alpinia officinarum is a rhizome belonging to the family zingeberaeceae. Hydro alcoholic extract by hot and cold maceration and methanol extract by percolation process Qualitative phytochemical analysis of extract of Alpinia officinarum rhizome showed a majority of the compound including tannins, alkaloids, flavonoids and saponins. Hydroalcoholic extract prepared by hot maceration process was found to contain more phenol and flavonol and it was measured as 50.1 mg/g and 54.02 mg/g, respectively. All the three extracts showed moderate to potent antimicrobial activity against the Bacillus cereus, Staphylococcus aureas, Pseudomonas auroginosa, Escherichia coli. None of the extracts showed antifungal activity against Aspergillus niger and Candida albicans. All the three extracts showed a concentration dependent radical scavenging activity by inhibiting diphenylpicrylhydrazyl free radical at the same time hydroalcoholic extract prepared by hot maceration process showed better reducing and total antioxidant activity.
Highlights
Alpinia officinarum is a rhizome belonging to the family zingeberaeceae
The free radical diphenyl picryl hydrazyl (DPPH) is reduced to the corresponding hydrazine when it reacts with hydrogen donors, this stability is evaluated by the decolouration assay which evaluates the decrease in absorbance at 518-528 nm produced by the addition of the antioxidant to a DPPH solution in ethanol or methanol
HA(HM) is hydroalcoholic extract prepared by hot maceration process, HA(CM) is hydroalcoholic extract prepared by cold maceration process and ME(PER) represents methanol extract prepared by percolation process. +indicates the presence and - indicates the absence
Summary
Alpinia officinarum is a rhizome belonging to the family zingeberaeceae. Alpinia officinarum is a rhizome belonging to the family, zingeberaeceae, cultivated in South East Asia. This rhizome is characterized by dark reddish brown colour which has a strong aromatic odour. The dried rhizomes of Alpinia officinarum were powdered and extracted with 50 % ethanol by hot and cold maceration. Evaluation of antimicrobial activity was performed by Cup plate method. In vitro antioxidant activity was evaluated by diphenyl picryl hydrazyl (DPPH) radical scavenging method. The principle of this assay is based on the measurement of the scavenging ability of the antioxidant towards the stable radical.
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