Abstract
Oxidative stress is implicated in human diseases including cancer or neurodegenerative diseases. On the other hand, lipid and microbial spoilage are the main issues of food degradation. Bioactive peptides with antioxidant and antimicrobial activity could solve both problems and create an opportunity to improve the sustainability of the meat industry. Recently, meat by-products are subject of numerous studies to produce antioxidant peptides, highlighting pork liver as a potential source of hydrolysates. To achieve this purpose, pork liver was digested with Flavourzyme at four reaction times (4, 6, 8, and 10 h) and filtered with cut-offs of 5, 10, and 30-kDa molecular weight. Monitoring hydrolysis with SDS-PAGE showed that the reaction was almost complete. Free amino acid profile exhibited that aliphatic and aromatic amino acids were released in a higher amount at longer reaction times. Heat map analysis demonstrated that a hydrolysis time beyond 6 h, displayed a differential amino acid pattern enabling us to optimize the enzymatic reaction. Antioxidant activity was assessed using ABTS, DPPH, FRAP, and ORAC tests, while antimicrobial assay was carried out against Gram-positive and Gram-negative. ABTS and DPPH values revealed that hydrolysates showed a high antioxidant capacity, as well as an inhibition of growth of Brochothrix thermosphata particularly 30 kDa hydrolysates.
Highlights
Oxidative stress reflects an unbalance between overproduction of reactive oxygen species (ROS)and the inability to counter or detoxify these molecules through the antioxidant mechanisms at cellular level [1]
The hydrolysis of the porcine liver was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
Several authors hypothesized that Flavourzyme contains a hydrolysis times
Summary
Oxidative stress reflects an unbalance between overproduction of reactive oxygen species (ROS)and the inability to counter or detoxify these molecules through the antioxidant mechanisms at cellular level [1]. Sci. 2020, 10, 3950 molecules accumulated in a deficient antioxidant defense system leading to oxidative stress [2]. In this context, ROS are capable of damaging DNA, lipids, and proteins causing alterations in membrane properties (permeability and stability) and negatively affecting cellular function [3,4,5]. ROS are capable of damaging DNA, lipids, and proteins causing alterations in membrane properties (permeability and stability) and negatively affecting cellular function [3,4,5] They play a pivotal role in the release of cytochrome and apoptosis induction [6]. Oxidative stress has been associated with several chronic diseases including asthma, atherosclerosis, cancer, rheumatoid arthritis, stroke, allergic, cardiovascular, and kidney and neurological diseases [1,7,8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
