Antioxidant and antimicrobial activity of lactic acid bacteria fermentation broth cultured on Acanthopanax koreanum extract

Abstract

Acanthopanax koreanum has been used in Korea to cure rheumatism, neuralgia, edema, dermatopathy, and impotence.1) Especially, this is cultivated only in Jeju. In this study, we heve investigated the antimicrobial and antioxidant activities on herb extract from Acanthopanax koreanum. Preparation of Acanthopnax koreanum used were a water extract of stems of theplant. The extracts of Acanthopnax koreanum originated from Jeju Island were provided from a stock farm product company(Jewoo Bio, Korea). Ferment strain was by four mixed strain(Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus plantrum, Bacillus subtilis) from 4.5brix concentration. The extract activity was compared at 7.5 brix, 4.5 brix and 4 mixed strains being concentrated liquid. We have tested the antioxidant activity by ESR(Electron Spin Resonance)2), DPPH(1,1-diphenyl-2-picrylhydrazyl) and hydroxyl radical activity. Four mixed strains culture extracts showed 40-89% of the DPPH radical scavenger activity and 25-78% ofthe hydroxyl radical scanvenger activity. The sensitivity of extracts was determined against different bacterial strains by disc diffusion method using 24h-old bacterial culture suspension (105CFU/ml) in agar media. The extracts exhibited antimicrobial activity against Vibrio alginolyticus KCCM 40513, Vibrio campbellii KCCM 41986, Vibrio harveyi KCCM 40866, Vibrio salmonicida KCCM 41663, Vibrio furnissii KCCM 41679, Vibrio anguillarum KCTC 2711, Streptococcus parauberis KCTC 3651, Streptococcus iniae KCTC 3657, Edwardsiella tarda KCTC 12267. The higher antimicrobial activity was observed on 4 mixed strains containing the 4.5brix extract.