Antioxidant and antimicrobial activities of different parts of Pancratium maritimum

Abstract

Pancratium maritimum L. (Amaryllidaceae) is grown in the Mediterranean regions shows a significant medicinal interest. This study was conducted to evaluate the antioxidant and antimicrobial activity of the extracts of Pancratium maritimum parts (root, bulb, leaves, flowers and seeds). The antioxidant activity was estimated by different methods. All the methanolic extracts of Pancratium maritimum parts exhibited good antioxidant activity. Flowers and leaves extracts recorded the highest DPPH radical scavenging activity (85.22%± 1.23 and 81.34%± 0.74) for flowers and leaves methanolic extract respectively. The highest Reducing power was found in flowers extract (0.844 ± 0.005 while the lowest (0.465 ± 0.004) was recorded with bulbs methanolic extract. Flowers extract exhibited the highest radical scavenging activity using ABTS assay (72.31%± 0.928). On the other hand bulbs extract had the superiority in metal chelating activity it recorded 76.92%± 1.33. Leaves extract contains the highest total phenolic and flavonoid contents (5.36 ± 0.082 mg gallic/g DW and 1.17 ± 0.03 mg quercetin/g DW respectively). Chloroform extract of all parts exhibited a strong fungicidal action against the selected multi-drug resistant Candida albicans [1]. Also the extract of roots showed strong activity against Gram-negative bacteria by Klebsiella pneumoniae (32 mm), Staphylococcus aureus (28 mm) and Salmonella typhi (23 mm), while the extracts of bulbs and seeds displayed strong activity against Salmonella typhi, Staphylococcus aureus (40 mm). Compound (1) was isolated from the leaves of Pancratium maritimum using different spectroscopic analysis (ESI-MS, 1D and 2D HNMR) and identified as N-cyanomethylnorboldine. Present results revealed that some parts of Pancratium maritimum have remarkable antioxidant and antimicrobial activity.