Antioxidant and Anti-Inflammatory Properties of Anacardium occidentale Leaf Extract.

Natália Cabral Souza,Daniel Pens Gelain,Matheus Augusto De Bittencourt Pasquali,Christina Da Silva Camillo,José Cláudio Fonseca Moreira,Ricardo D’Oliveira Albanus,Silvana Maria Zucolotto Langassner,Juliana Medeiros De Oliveira,Maurílio Da Silva Morrone,Rodrigo Juliani Siqueira Dalmolin,Maria Do Socorro Medeiros Amarante

doi:10.1155/2017/2787308

Abstract

In tropical America, principally in Northeastern Brazil, the leaf extract of Anacardium occidentale is traditionally used for treatment of different diseases. However, chemical and biological properties and activities of Anacardium occidentale are poorly investigated and known. Here, we evaluated the antioxidant and anti-inflammatory activities “in vitro” of leaf extract from Anacardium occidentale. Our results show that leaf extract exhibits antioxidant activity when used to treat RAW 264.7 macrophage cells. Antioxidant effects were observed by decrease in oxidative damage in macrophage cells treated with 0.5 µg/mL and 5 µg/mL of leaf extract. Moreover, leaf extract reversed oxidative damage and inflammatory parameters induced in LPS-stimulated RAW 264.7 macrophage cells. Leaf extract at 0.5 µg/mL and 5 µg/mL was able to inhibit release of TNF-α and IL-1β in LPS-stimulated cells. Taken together, our results indicate antioxidant and anti-inflammatory effects of leaf extract from Anacardium occidentale and reveal the positive effects that intake of these products can mediate in biological system.

Highlights

Anacardium occidentale is a tropical plant that occurs principally in Northeastern Brazil [1]
RAW 264.7 macrophage cells were treated with Anacardium occidentale extract at concentrations of 0.5 μg/mL, 5 μg/mL, and 500 μg/mL (Figure 1) for determination of viability
The levels of lipid peroxidation and carbonylation of proteins decreased at concentrations of 0.5 μg/mL and 5 μg/mL (Table 1) when compared to the control group in cells treated with leaf extract for 24 hours

Summary

Introduction

Anacardium occidentale is a tropical plant that occurs principally in Northeastern Brazil [1]. Other important effect of AAs is regulating the activity of histone deacetylases (HDACs) [9] These are responsible for modulating gene expression in cells, and can be involved in cellular differentiation and proliferation. Another effect recently demonstrated is the modulation of NF-κB activity in cells treated with extracts from Anacardium occidentale [7]. The extract was able to mediate inhibition of proinflammatory cytokines secretion such as IL-1β and TNFα in lipopolysaccharides- (LPS-) stimulated macrophages cells These data provide evidence that leaf extract may exert an antioxidant and anti-inflammatory role due to the properties of biomolecules presents in the extract

Materials and Methods

Results

Discussion