Abstract
Purpose: To investigate the antioxidant and antihaemolytic properties of the leaves of Laser trilubum grown in Gaduk, Iran. Methods: The antioxidant and antihaemolytic activities of the hydroalcohol extract of L. trilobum L. leaf were investigated by haemoglobin-induced linoleic acid peroxidation, scavenging of 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, nitric oxide and hydrogen peroxide, as well as by assessment of reducing power and Fe2+ chelating activity, using standard methods. Phenol and flavonoid contents were determined as gallic acid and quercetin equivalents, respectively. Results: The extract showed antioxidant activity in some models, with 50 % inhibitory concentration (IC50) for DPPH radical-scavenging activity being 147.1 ± 7.5 μg ml-1. The extract showed good nitric oxide-scavenging activity of between 0.1 and 1.6 mg ml-1 (IC50 = 517.7 ± 23.1 vs. 20 ± 0.01 μg ml-1 for quercetin), weak Fe2+ chelating ability (IC50, 906.9 ± 37.8 μg ml-1), and low antioxidant activity in haemoglobin-induced linoleic acid system. However, it was capable of scavenging hydrogen peroxide in a concentration-dependent manner while also exhibiting potent antihaemolytic activity against H2O2 - induced haemolysis (IC50, 169.6 ± 6.9 μg ml-1). Total phenolic content was 75 ± 3 mg gallic acid equivalent/g and total flavonoid content 59.2 ± 2.1 mg quercetin equivalent/g. The amounts of gallic acid, quercetin and rutin were 0.99 ± 0.04, 0.63 ± 0.01 and < 0.10 μg/mg, respectively. Conclusion: L. trilobum exhibited good but varying levels of antioxidant and antihaemolytic activities in nearly all the models studied, when compared with controls.Keywords: Antioxidant activity, Laser trilobum, Antihaemolytic, Flavonoids, Kefe cumin
Highlights
The pathology of numerous chronic diseases, including cancer and heart disease, involves oxidative damage to cellular components [1]
1 ml of sodium nitroprusside (10 mM) in phosphate-buffered saline was mixed with varying concentrations of Laser trilubum extract (100 - 1600 μg ml-1) dissolved in water and incubated at room temperature for 150 min
The extract scavenged hydrogen peroxide in a concentration-dependent manner with IC50 of 300 ± 14 μg ml-1 compared with IC50 values of 21.4 ± 1.1 and 52.0 ± 2.6 μg ml-1 for ascorbic acid and quercetin, respectively (Table 2)
Summary
The pathology of numerous chronic diseases, including cancer and heart disease, involves oxidative damage to cellular components [1]. 0.5 ml solution of the extract in methanol (1.6 mg ml-1) was separately mixed with 1.5 ml of methanol, 0.1 ml of 10 % aluminum chloride, 0.1 ml of 1M potassium acetate and 2.8 ml of distilled water, and left at room temperature for 30 min. EDTA (6.25 - 100 μg ml-1) was used as the standard In this experiment, 1 ml of sodium nitroprusside (10 mM) in phosphate-buffered saline was mixed with varying concentrations of Laser trilubum extract (100 - 1600 μg ml-1) dissolved in water and incubated at room temperature for 150 min. The mixture was centrifuged at 3000 rpm for 10 min and 2.5 ml of the upper layer of the solution was mixed with distilled 2.5 ml of water and 0.5 ml of 0.1 % FeCl3, and the absorbance was measured spectrophotometrically at 700 nm.
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