Antioxidant and antiglycation properties of extracts of Diplotaxis tenuifolia

Abstract

The goal of this study was to investigate phenolic contents and biological activities in leaves of Diplotaxis tenuifolia collected in Patagonia (Argentina). Anti-glycation activities were tested by bovine serum albumin-glucose (BSA-glu) and Maillard reaction models. Only in the case of water extract of dry matter, Maillard reaction could be 40 % inhibited. The remaining results do not allow make definitive conclusions. Water extracts exhibited higher polyphenol content than methanolic ones (1074.25 and 647.5 mg Gallic acid equivalent/100 g of dry matter, respectively). On the other hand, flavonoid content was comparable between the water and methanolic extracts (218.2 and 213.4 mg quercetin equivalent/100 g of dry matter, respectively). Regarding antioxidant properties, CUPRAC (Cupric reducing antioxidant capacity) showed values of 655.6 mg Trolox equivalent/100 g of dry matter and 749.6 mg ascorbic acid equivalent/100 g of dry matter for water extracts and 1045,2 mg Trolox equivalent/100 g of dry matter and 1203,9 mg ascorbic acid equivalent/100 g of dry matter for methanolic ones. DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay displayed values of 203,3 mg Trolox equivalent/100 g of dry matter and 180.0 mg ascorbic acid equivalent/100 g of dry matter for water extracts, and 97.7 mg Trolox equivalent/100 g of dry matter and 89,3 mg ascorbic acid equivalent/100 g of dry matter for methanolic ones. The results obtained highlight the antioxidative potential of D. tenuifolia collected in extreme regions like Patagonia. This edible plant is an antioxidant-rich source that should be considered as functional food, its consumption is recommendable to prevent cell damage by scavenging deleterious free radicals.