Antioxidant and Anticancer Potential of Raja Bulu Banana Peel and Heart (Musa acuminata Colla (AAB group)) Ethanol Extracts in MCF-7 Cell Lines

Afifah Bambang Sutjiatmo,Alya Mardhotillah Azizah,Ida Sumiati,Wahyu Widowati,Twice Priestu,Hanna Sari Widya Kusuma,Seila Arumwardana

doi:10.22146/mot.55333

Abstract

Breast cancer is an uncontrolled cell growth in breast tissue. Surgical treatments of breast cancer can reduce breast aesthetics and chemotherapy can cause severe side effects. It makes the searches for plants as breast anticancer agents intensively carried out. Several studies have shown that banana peels and hearts possess antioxidant and anticancer activity. This study aims to determine the fruit peel and heart of Raja Bulu banana (Musa acuminata Colla (AAB group)), an endemic banana species in Indonesia, potential as antioxidant and anticancer agent in MCF-7 cells. Antioxidant potential was determined by using 2,2-diphenyl-1picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging activity assay. Anticancer potential was determined by cytotoxic test using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The results showed that 70% ethanol extract of fruit peel (PBEE) and heart (HBEE) of Raja Bulu banana had median inhibition concentration (IC50) for DPPH scavenging activity at 115.32 µg/mL and 162.52 µg/mL respectively, while for H2O2 scavenging activity at 624.80 µg/mL and 497.13 µg/mL respectively. Anticancer potential was expressed by inhibiting concentration of 50% proliferation (IC50) of MCF-7 cells for PBEE and HBEE were 115.001 µg/mL and 338.469 µg/mL respectively. This study showed that PBEE and HBEE have antioxidant and anticancer.

Highlights

Breast cancer is a malignancy in breast epithelial tissue that can originate from the breast ducts and breast glands (National Cancer Institute, 2009)
Based on previous research on antioxidant activity and anticancer screening of banana peels in n-hexane, ethanol, and water extracts against HUVEC, HCT-116, and MCF-7 cells was shown that the highest ability to inhibit MCF-7 cell growth was found in 12.07% ethanol extract of banana peels (Dahham et al, 2016)
The antioxidant and anticancer potential of 70% ethanol extract Raja Bulu banana (Musa acuminata Colla (AAB group)) peel and blossom in MCF-7 cells was determined in this study

Summary

INTRODUCTION

Breast cancer is a malignancy in breast epithelial tissue that can originate from the breast ducts (lactiferous ductus) and breast glands (lobules) (National Cancer Institute, 2009). Besides cancer risk could be increased by post-translational protein modifications or mutations in cancer-related genes by some reaction like nitration, nitrosation, phosphorylation, acetylation, and poly (ADPribosyl) ation. It occurs as the result of free radicals or lipid peroxidation byproducts presence such as malondialdehyde (MDA) and 4-hydroxynonenal (4HNE). Herbal medicine was containing antioxidants that could be the sources for replacing synthetic chemotherapeutic agents. It has advantages where they are less cytotoxic toward healthy cells safer to use (Widowati et al, 2013a). The antioxidant and anticancer potential of 70% ethanol extract Raja Bulu banana (Musa acuminata Colla (AAB group)) peel and blossom in MCF-7 cells was determined in this study

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