Antioxidant and antibacterial evaluation of leaf extracts of Prunus cerasoides Buch.-Ham. ex D.Don; An In Vitro and In Silico study

Abstract

BackgroundMany infectious bacteria have changed throughout time and there are alarmingly large number of antibiotic resistant species that can withstand the inhibitory impact of these treatments., Plants are new sources of antibacterial agents hence there is a need to determine and evaluate the antioxidant activity, antibacterial properties of medicinal plants ObjectiveThe present study focuses on qualitative, quantitative phytochemical analysis, antioxidant, and antimicrobial evaluation of leaves’ extracts of Prunus cerasoides Buch. -Ham. ex D. Don. Materials and methodsThe Antioxidant activity of leaves extracts of Prunus cerasoides Buch. -Ham. ex D.Don was determined by DPPH method. Antibacterial activity was determined by serial dilution method. Further docking interactions were investigated using Maestro 12.7 (Schrodinger 2021) and ADME by using QikProp tool of Schrodingers 2021. ResultsThe leaves’ extracts of Prunus cerasoides Buch. -Ham. ex D.Don showed potent antioxidant and antibacterial activities. Methanol extract was found to be the most active against all the four strains of bacteria and showed MIC value (3.125 μg/mL) against both E. coli and S. aureus. Ethyl acetate and methanol extracts had the highest radical scavenging activity. Further molecular docking and ADME studies for phytoconstituents of Prunus cerasoides Buch. -Ham. ex D. Don. using different bacterial protein targets of reported phytoconstituents of Prunus cerasoides Buch. -Ham. ex D. Don. and found that emodin, amygdalin and aromadendrin were the phytoconstituents having good docking score and drug likeliness properties. ConclusionsThe leaves extracts of Prunus cerasoides Buch. -Ham. ex D.Don showed potent free radical scavenging property as well as antibacterial activity.