Antioxidant and Anti-inflammatory Mediated Mechanism in Thermal Wound Healing by Gel Containing Flower Extract of Butea monosperma

Abstract

Burn injury produces reactive oxygen species which causes inflammatory consequences in the exposed tissue. Antioxidant components widely used to scavenge reactive oxygen species as well prevents the inflammatory consequences. The objective of the present study was to evaluate thermal wound healing activity of gel containing the flower extract of Butea monosperma (FEBM). Fifty adult, male Wistar-albino rats were divided into five groups of ten animals (five animals each for 10 and 20 day trials period). A burn wound was induced on the back side of all the rats. The respective burned areas in all groups of animals were treated with blank (control), standard silver sulphadiazeine (SSD), gels of flower extract of B. monosperma (0.5, 1 and 1.5 %). After 10 and 20 day trial periods, the rats were sacrificed and the wound samples were collected for determination of lipid peroxidation and histopathological examinations. The results of treated wound showed much faster healing as compared to control. The significant effect on wound closure was observed with 1.5 % gel at 20 day which was comparable with SSD. The result of lipid peroxidation indicated the anti-lipid peroxidation activity of gels containing FEBM. Results also showed in vitro free radicals scavenging activity and anti-inflammatory activity which may be due to the presence of flavonoids and thus FEBM provides the beneficial effects in the acceleration of wound healing. In conclusion, topical applications of gels containing the flower extract of B. monosperma are effective in healing of burn related skin wounds in the rat model.