Abstract
Oxidative stress is a phenomenon caused by an imbalance between the production of reactive oxygen species and antioxidant defenses. It plays an important role in numerous disease states, including chronic kidney disease, neurological disorders, cardiovascular diseases, diabetes, and cancer. Lactic acid bacteria (LAB) are known to have prominent antioxidant properties. Therefore, this study aimed to measure the antioxidant activity and anti-inflammatory potential of LAB isolated from animals for the efficient use of probiotics with host specificity. Antioxidant activity measurements of sixteen strains revealed that ABTS radical scavenging activities ranged from 26.3 to 57.4%, and DPPH free radical scavenging activities ranged from 4.7 to 13.5%. Based on the antioxidant activity assessment, five strains (Enterococcus faecium MG9003(YH9003), Enterococcus faecium MG9007(YH9007), Lactobacillus reuteri MG9012(YH9012), Lactobacillus fermentum MG9014(YH9014), and Pediococcus pentosaceus MG9015(YH9015)) were selected with the consideration of fermentation productivity (>1 × 109 CFU/g). The selected strains exhibited nitric oxide inhibition and inhibited inducible nitric oxide synthase and cyclooxygenase expression. Furthermore, probiotic properties, including intestinal adhesion and stability, were identified. Our results show that the selected animal-derived strains can be effective probiotic candidates for potential effects on animal hosts.
Highlights
Enstrains were identified as three species belonging to the Pediococcus, Lactobacillus, terococcus genera, including one P. pentosaceus strain, four P. acidilactici strains, one L. and aniEnterococcus genera, includingstrains, one P.two pentosaceus strain, four P.one acidilactici one malis strain, two
The results show that the inducible NOS (iNOS) (Figure 4b) and COX-2 expression levels (Figure 4c) were increased in LPS-induced
This study was performed to evaluate the antioxidant and anti-inflammatory activity and identify the probiotic properties of novel Lactic acid bacteria (LAB) strains derived from animal hosts
Summary
Probiotics are live microorganisms with a positive influence on the balance of gastrointestinal microorganisms of their host when ingested in appropriate amounts [1] They act as antioxidants through the ability to chelate metal ion, the generation of metabolites such as lactate, downregulation of reactive oxygen species (ROS)-producing enzymes, and upregulation of antioxidant activities [2]. These mechanisms improve the host’s defense against oxidative stress and contribute to the prevention of various diseases, including digestive disorders and cancer [3]
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