Antioxidant and Anti-Aging Activity of Green Mustard Ethanol Extract Gel

Abstract

The skin is the organ of the body that is frequently exposed to direct UV rays from the sun, which causes the formation of ROS (reactive oxygen species), leading to cell death and tissue damage. This health problem can be overcome by using antioxidants to stabilize the free radicals. Mustard as an organic substance which contains polysaccharide compounds, vitamin C, carotene, quercetin, routine compounds, kaempferol and β-cytostreol, all of these are beneficial to skin health and beauty. Therefore, this study aims to determine the antioxidant activity of the total flavonoids and phenol content of mustard ethanol extract using the DPPH method. Furthermore, the extract at concentrations of 2, 4, and 6% was also tested for its anti-aging activity using the following parameters: moisture and oil content, texture, collagen, wrinkles, pigment, sensitivity, and pores. The results showed that the antioxidant activity of the green mustard had an IC50 value of 170.7839 µg/ml, a total phenol content of 14.471 mg GAE/g extract and flavonoid content of 12.753 mg QE/g extract. The effectiveness of the aging activity of the extract was tested using formulation 6%, which is better than 2%, and 4%. Thus, the percentage of water content recovery, oil content, texture, collagen, wrinkles, stains, sensitivity and pores respectively was 36.41, 39.33, 64.07, 17.65, 65.28, 50.14, 49.10 and 35.09%. Consequently, it was concluded that the extract has the potential of being developed into a herbal beauty product.