Antioxidant, analgesic, anti-inflammatory, and antipyretic potentialities of leaves of Artabotrys hexapetalus, established by in silico analysis

Abstract

BackgroundArtabotrys hexapetalus is a rare plant in the Indian subcontinent. This plant is used for different therapeutic purposes in folk medicine and many bioactive compounds are reported in this plant. Considering its traditional uses and the presence of medicinal compounds, we aimed to evaluate some pharmacological properties of its leaves along with in silico analysis in scientific ways. MethodsAntioxidant tests were conducted in both qualitative and quantitative ways. 2,2-Diphenyl-1-picryl hydrazyl (DPPH), hydrogen peroxide, and superoxide radicals scavenging with FeCl3 reducing properties of A. hexapetalus extract were carried out. Acetic acid-induced writhing, tail immersion, formalin-induced paw edema, and Brewer's yeast-induced pyrexia methods were followed to conduct peripheral analgesic, central analgesic, anti-inflammatory, and antipyretic potentialities, respectively. Finally, molecular docking and absorption, distribution, metabolism, excretion, and toxicity (ADMET) analysis of reported compounds were conducted. ResultsThe A. hexapetalus extract was found to possess a quite good amount of phenolics, flavonoids, and tannins. This extract also revealed good radical scavenging effects along with ferric chloride-reducing capabilities. In the analgesic tests, the extract showed excellent responses in writhing inhibition and elongation in tail withdrawal time. The extract also significantly lessened paw edema over time at both doses. A notable decrease in body temperature was also observed. Those responses revealed nice pharmacological potentialities. Along with these findings and conducting in silico analysis, we finally proposed seven molecules as lead compounds. ConclusionOur analysis revealed that A. hexapetalus leaves are highly enriched with antioxidants and the extract showed good pharmacological characteristics.