Antioxidant Activity Test of Red Pidada Leaves (Sonneratia caseolaris L.) using ABTS Method (2,2-azinobis-(3-ethylbenzothiazolin)-6-sulfonicacid)

Abstract

The Red Pidada (Sonneratia caseolaris L.) is one of the plants used in traditional ways, especially by Borneo's people, where the red Pidada leaves are used as a cool powder composition and applied to the face while doing activities under exposure to sunlight over a long time. Red Pidada extract has a very strong antioxidant activity of 21.62 ppm because it has secondary metabolite, which is a medicinal ingredients and have a strong molecular structure, and have various biological activities. Pidada redplant (Sonneratia caseolaris L.) is a mangrove that has antioxidant potential. Free radicals can cause cancer, heart disease, premature aging, and other degenerative diseases. The research objective was to determine the antioxidant activity of the Pidada red leaf extract and fraction.The research was conducted qualitatively and quantitatively. The qualitative test was carried out by the colour change test and the quantitative test using the ABTS method (2,2-azinobis-3-ethyl benzothiazolin-6-sulfonic acid) with a UV-Visspectrophotometer. Pidada redleaves were extracted and fractionated, namely ethanol extract, n-hexane fraction, ethyl acetate fraction, and ethanol fraction, and made inti various concentrations compared to vitamin C.The IC50 value determined the antioxidant activity. The results showed that the ethanol extract had an IC50 value of 1.32 ppm (very strong), n-hexanefraction of 19.89 ppm (very strong), ethylacetate fraction of 0.50 ppm (very strong), and ethanolfraction of 1.63 ppm (very strong).