Abstract

The antioxidative activity of the 80% ethanol extract obtained from eleven commonly consumed wild edible plants was determined according to the phosphomolybdenum method, reducing power, metal chelating, superoxide anion and free radical scavenging activity and compared to standard compounds such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and trolox. Total phenolic compounds, flavonoids, and anthocyanins in the extracts were also measured. Trachystemon orientalis, Vaccinium mrytillus, Rumex acetosella Polygonum amphibium, Beta vulgaris, and Similax Excelsa had the highest antioxidant capacities. Overall results showed that these plants can serve as good sources of bioactive polyphenols in the human diet and can be regarded as good candidates for nutritional supplement formulations due to their high concentrations of total phenolic compounds, flavonoids and anthocyanins as well as their strong antioxidant activity.

Highlights

  • Reactive oxygen molecules (ROS) such as superoxide (O2.Ϫ), hydroxyl (OH.Ϫ) and peroxyl (ROO.Ϫ) radicals are generated in a situation of oxidative stress

  • The antioxidant activities of Amaranthus retroflexus, Tussilago farfara, Trachystemon orientalis, Beta Vulgaris, Brassica oleracea, Vaccinium mrytillus, Ornithogalum umbellatum, Polygonum amphibium, Rumex acetosella, Similax excelsa and Chaerophyllum byzantinum were determined using the phosphomolybdenum method, reducing power, metal chelating, superoxide anion and free radical scavenging activity. Another aim was to evaluate whether the total phenolic, flavonoid and anthocyanin contents of wild edible plants were correlated with antioxidant activity

  • The total phenolic compounds, total flavonoids and the total anthocyanins were measured for all the samples

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Summary

SUMMARY

The antioxidative activity of the 80% ethanol extract obtained from eleven commonly consumed wild edible plants was determined according to the phosphomolybdenum method, reducing power, metal chelating, superoxide anion and free radical scavenging activity and compared to standard compounds such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and trolox. Flavonoids, and anthocyanins in the extracts were measured. Overall results showed that these plants can serve as good sources of bioactive polyphenols in the human diet and can be regarded as good candidates for nutritional supplement formulations due to their high concentrations of total phenolic compounds, flavonoids and anthocyanins as well as their strong antioxidant activity. KEY-WORDS: Antioxidant activity – Anthocyanins – Black Sea Region – Flavonoids – Total phenolic compounds – Wild edible plants

INTRODUCTION
Chemicals
Plant materials
Amount of total phenolic compounds
Chaerophyllum byzantinum Umbelliferae Boiss
Amount of flavonoids
Amount of anthocyanins
Determination of total antioxidant activity
Assay of reducing power
Metal chelating activity
Assay of superoxide anion scavenging activity
2.10. Assay of free radical scavenging activity
2.11. Statistical analysis
RESULTS AND DISCUSSION
11 Vaccinium mrytillus
Total antioxidant activity
Reducing power
Superoxide anion scavenging activity
Free radical scavenging activity
CONCLUSION
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